Full Product Name
MAN1 Mouse mAb
Product Synonym Names
Inner nuclear membrane protein Man1; integral inner nuclear membrane protein; integral inner nuclear membrane protein MAN1; LEM domain-containing protein 3; MAN1; AI316861; LEMD3; MAN1
Product Gene Name
anti-MAN1 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9Y2U8
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human MAN1
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Other Notes
Small volumes of anti-MAN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MAN1 antibody
This locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis.
Product Categories/Family for anti-MAN1 antibody
Cell Marker; Subcellular Marker; Nucleoplasma
Applications Tested/Suitable for anti-MAN1 antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-MAN1 antibody
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200
Western Blot (WB) of anti-MAN1 antibody
Immunoprecipitation (IP) of anti-MAN1 antibody
MAN1 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25114 of 10 ug. Western blot was performed from the immunopre-cipitate using M25114 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.
Predicted band size : 100 KDa
Observed band size : 100 KDa
Blocking and dilution buffer and concentration:5% milk/TBST.
Flow Cytometry (FC/FACS) of anti-MAN1 antibody
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling MAN1 with M25114 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.
NCBI/Uniprot data below describe general gene information for MAN1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001161086.1
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NCBI GenBank Nucleotide #
NM_001167614.1
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UniProt Primary Accession #
Q9Y2U8
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UniProt Secondary Accession #
Q9NT47; Q9NYA5[Other Products]
UniProt Related Accession #
Q9Y2U8[Other Products]
Molecular Weight
Predicted MW: 100kDa
NCBI Official Full Name
inner nuclear membrane protein Man1 isoform 2
NCBI Official Synonym Full Names
LEM domain containing 3
NCBI Official Symbol
LEMD3??[Similar Products]
NCBI Official Synonym Symbols
MAN1
??[Similar Products]
NCBI Protein Information
inner nuclear membrane protein Man1
UniProt Protein Name
Inner nuclear membrane protein Man1
UniProt Synonym Protein Names
LEM domain-containing protein 3
Protein Family
Mannan endo-1,4-beta-mannosidase
UniProt Gene Name
LEMD3??[Similar Products]
UniProt Synonym Gene Names
MAN1??[Similar Products]
NCBI Summary for MAN1
This locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis.[provided by RefSeq, Nov 2009]
UniProt Comments for MAN1
Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest.
Research Articles on MAN1
1. studies demonstrated that lower levels of MAN1 in differentiating MSC are associated with higher osteogenesis and lower adipogenesis. High levels of MAN1 only affected adipogenesis.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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