For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of ANTXR2. No significant cross-reactivity or interference between ANTXR2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANTXR2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ANTXR2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7240722 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anthrax toxin receptor 2 (ANTXR2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ANTXR2. The ELISA analytical biochemical technique of the MBS7240722 kit is based on ANTXR2 antibody-ANTXR2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ANTXR2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ANTXR2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This ANTXR2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ANTXR2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: ANTXR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANTXR2 antibody and an ANTXR2-HRP conjugate. The assay sample and buffer are incubated together with ANTXR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANTXR2 concentration since ANTXR2 from samples and ANTXR2-HRP conjugate compete for the anti-ANTXR2 antibody . Since the number of sites is limited, as more sites are occupied by ANTXR2 from the sample, fewer sites are left to bind ANTXR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANTXR2 concentration in each sample is interpolated from this standard curve.

Neurobiology

53,493 Da

anthrax toxin receptor 2

anthrax toxin receptor 2; capillary morphogenesis protein 2; capillary morphogenesis gene 2 protein

Anthrax toxin receptor 2

CMG2; CMG-2??[Similar Products]

ANTR2_HUMAN

This gene encodes a receptor for anthrax toxin. The protein binds to collagen IV and laminin, suggesting that it may be involved in extracellular matrix adhesion. Mutations in this gene cause juvenile hyaline fibromatosis and infantile systemic hyalinosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

ANTXR2: Necessary for cellular interactions with laminin and the extracellular matrix. Defects in ANTXR2 are the cause of infantile systemic hyalinosis (ISH). This autosomal recessive syndrome is similar to JHF, but has an earlier onset and a more severe course. Symptoms appear at birth or within the first months of life, with painful, swollen joint contractures, osteopenia, osteoporosis and livid red hyperpigmentation over bony prominences. Patients develop multiple subcutaneous skin tumors and gingival hypertrophy. Hyaline deposits in multiple organs, recurrent infections and intractable diarrhea often lead to death within the first 2 years of life. Surviving children may suffer from severely reduced mobility due to joint contractures. Defects in ANTXR2 are the cause of juvenile hyaline fibromatosis (JHF). JHF is an autosomal recessive syndrome that is similar to ISH but takes a milder course. It is characterized by hyaline deposition in the dermis, multiple subcutaneous skin tumors and gingival hypertrophy, followed by progressive joint contractions, osteopenia and osteoporosis that may lead to a severe limitation of mobility. Belongs to the ATR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q21.21

Cellular Component: endoplasmic reticulum membrane; extracellular region; integral to membrane; plasma membrane; endosome membrane; external side of plasma membrane

Molecular Function: protein binding; metal ion binding; receptor activity

Biological Process: reproductive process; pathogenesis

Disease: Hyaline Fibromatosis Syndrome

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