Full Product Name
PPT1 siRNA (Mouse)
Product Synonym Names
PPT; Palmitoyl-protein thioesterase 1; PPT-1; Palmitoyl-protein hydrolase 1
Product Gene Name
PPT1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O88531
Specificity
PPT1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PPT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PPT1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PPT1 sirna
siRNA to inhibit PPT1 expression using RNA interference
Applications Tested/Suitable for PPT1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PPT1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_032943.2
[Other Products]
NCBI GenBank Nucleotide #
NM_008917.3
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UniProt Primary Accession #
O88531
[Other Products]
UniProt Secondary Accession #
Q9D681; Q9WTY3[Other Products]
UniProt Related Accession #
O88531[Other Products]
Molecular Weight
34,490 Da
NCBI Official Full Name
palmitoyl-protein thioesterase 1
NCBI Official Synonym Full Names
palmitoyl-protein thioesterase 1
NCBI Official Symbol
Ppt1??[Similar Products]
NCBI Official Synonym Symbols
PPT; CLN1; INCL; C77813; AA960502; D4Ertd184e; 9530043G02Rik
??[Similar Products]
NCBI Protein Information
palmitoyl-protein thioesterase 1
UniProt Protein Name
Palmitoyl-protein thioesterase 1
UniProt Synonym Protein Names
Palmitoyl-protein hydrolase 1
Protein Family
Palmitoyl-protein thioesterase
UniProt Gene Name
Ppt1??[Similar Products]
UniProt Synonym Gene Names
Ppt; PPT-1??[Similar Products]
UniProt Entry Name
PPT1_MOUSE
UniProt Comments for PPT1
PPT1: Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons. Defects in PPT1 are the cause of neuronal ceroid lipofuscinosis type 1 (CLN1). A form of neuronal ceroid lipofuscinosis with variable age at onset. Infantile, late- infantile, juvenile, and ***** onset have been reported. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN1 is referred to as granular osmiophilic deposits (GROD). Belongs to the palmitoyl-protein thioesterase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid Metabolism - fatty acid elongation in mitochondria; Hydrolase; EC 3.1.2.22
Cellular Component: Golgi apparatus; extracellular space; neuron projection; lysosome; dendrite; extracellular region; lipid raft; synaptic vesicle; membrane; cell soma; axon; synapse; nucleus
Molecular Function: hydrolase activity; palmitoyl-(protein) hydrolase activity; palmitoyl-CoA hydrolase activity
Biological Process: grooming behavior; nervous system development; pinocytosis; receptor-mediated endocytosis; regulation of phospholipase A2 activity; ***** locomotory behavior; neurotransmitter secretion; positive regulation of pinocytosis; positive regulation of receptor-mediated endocytosis; protein transport; visual perception; lysosome organization and biogenesis; cellular protein catabolic process; lysosomal lumen acidification; cofactor metabolic process; negative regulation of neuron apoptosis; negative regulation of cell growth; cofactor transport; brain development; lipid raft organization and biogenesis; lipid catabolic process; associative learning; negative regulation of apoptosis; cellular macromolecule catabolic process; protein depalmitoylation
Research Articles on PPT1
1. Data (including data from knockout mice) suggest that deficiency of Ppt1 leads to accumulation of granular osmiophilic deposits in many cell types, especially in astrocytes. [review-like article]
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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