Full Product Name
MC1R siRNA (Human)
Product Synonym Names
MSHR; Melanocyte-stimulating hormone receptor; MSH-R; Melanocortin receptor 1; MC1-R
Product Gene Name
MC1R sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q01726
Specificity
MC1R siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MC1R gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of MC1R sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MC1R sirna
siRNA to inhibit MC1R expression using RNA interference
Applications Tested/Suitable for MC1R sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for MC1R. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002377.4
[Other Products]
NCBI GenBank Nucleotide #
NM_002386.3
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UniProt Primary Accession #
Q01726
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UniProt Secondary Accession #
Q66K38; Q6UR93; Q8WWX6; Q8WWX7; Q96I33; Q96RU4; Q9UBF7; Q9UN58; Q9UN59; Q9UN60; Q9UN61[Other Products]
UniProt Related Accession #
Q01726[Other Products]
Molecular Weight
34,706 Da
NCBI Official Full Name
melanocyte-stimulating hormone receptor
NCBI Official Synonym Full Names
melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor)
NCBI Official Symbol
MC1R??[Similar Products]
NCBI Official Synonym Symbols
CMM5; MSH-R; SHEP2
??[Similar Products]
NCBI Protein Information
melanocyte-stimulating hormone receptor
UniProt Protein Name
Melanocyte-stimulating hormone receptor
UniProt Synonym Protein Names
Melanocortin receptor 1; MC1-R
Protein Family
Melanocyte-stimulating hormone receptor
UniProt Gene Name
MC1R??[Similar Products]
UniProt Synonym Gene Names
MSHR; MSH-R; MC1-R??[Similar Products]
UniProt Entry Name
MSHR_HUMAN
NCBI Summary for MC1R
This intronless gene encodes the receptor protein for melanocyte-stimulating hormone (MSH). The encoded protein, a seven pass transmembrane G protein coupled receptor, controls melanogenesis. Two types of melanin exist: red pheomelanin and black eumelanin. Gene mutations that lead to a loss in function are associated with increased pheomelanin production, which leads to lighter skin and hair color. Eumelanin is photoprotective but pheomelanin may contribute to UV-induced skin damage by generating free radicals upon UV radiation. Binding of MSH to its receptor activates the receptor and stimulates eumelanin synthesis. This receptor is a major determining factor in sun sensitivity and is a genetic risk factor for melanoma and non-melanoma skin cancer. Over 30 variant alleles have been identified which correlate with skin and hair color, providing evidence that this gene is an important component in determining normal human pigment variation. [provided by RefSeq, Jul 2008]
UniProt Comments for MC1R
MC1R: Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Genetic variations in MC1R are a cause of susceptibility to cutaneous malignant melanoma type 5 (CMM5). Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites. Belongs to the G-protein coupled receptor 1 family.
Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral
Chromosomal Location of Human Ortholog: 16q24.3
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: protein binding; hormone binding; melanocortin receptor activity; ubiquitin protein ligase binding; melanocyte stimulating hormone receptor activity; peptide receptor activity, G-protein coupled
Biological Process: melanin biosynthetic process; G-protein signaling, coupled to cyclic nucleotide second messenger; positive regulation of protein kinase B signaling cascade; pigmentation; UV protection; multicellular organismal development; positive regulation of cAMP biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; sensory perception of pain
Disease: Albinism, Oculocutaneous, Type Ii; Increased Analgesia From Kappa-opioid Receptor Agonist, Female-specific; Melanoma, Cutaneous Malignant, Susceptibility To, 5; Skin/hair/eye Pigmentation, Variation In, 2
Research Articles on MC1R
1. MC1R variants were not associated with both malignant melanoma and Parkinson disease.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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