For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of CTDP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS726389 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CTD Phosphatase 1 (CTDP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CTDP1. The ELISA analytical biochemical technique of the MBS726389 kit is based on CTDP1 antibody-CTDP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CTDP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CTDP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This CPN IGM ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofCPN IGM in the sample, thisCPN IGM ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusCPN IGM concentration. The concentration ofCPN IGM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This CPN IGM enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forCPN IGM. Standards or samples are then added to the microtiter plate wells andCPN IGM if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofCPN IGM present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forCPN IGM are added to each well to "sandwich" theCPN IGM immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containCPN IGM and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

93,485 Da

CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1

RNA polymerase II subunit A C-terminal domain phosphatase; serine phosphatase FCP1a; TFIIF-associating CTD phosphatase 1; CTD of POLR2A, phosphatase of, subunit 1; transcription factor IIF-associating CTD phosphatase 1

RNA polymerase II subunit A C-terminal domain phosphatase

FCP1??[Similar Products]

CTDP1_HUMAN

This gene encodes a protein which interacts with the carboxy-terminus of the RAP74 subunit of transcription initiation factor TFIIF, and functions as a phosphatase that processively dephosphorylates the C-terminus of POLR2A (a subunit of RNA polymerase II), making it available for initiation of gene expression. Mutations in this gene are associated with congenital cataracts, facial dysmorphism and neuropathy syndrome (CCFDN). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

CTDP1: a phospho-serine/threonine protein phosphatase which interacts with the carboxy-terminus of transcription initiation factor TFIIF-alpha, a transcription factor which regulates elongation as well as initiation by RNA polymerase II. Its phosphorylation increases binding to TFIIF-alpha, affecting transcription elongation. Alternative splicing results in two different isoforms.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor); Transcription initiation complex

Chromosomal Location of Human Ortholog: 18q23

Cellular Component: nucleoplasm; spindle pole; centrosome; cytoplasm; spindle; midbody; spindle midzone; actin cytoskeleton

Molecular Function: DNA-directed RNA polymerase activity; CTD phosphatase activity

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; cell division; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression; protein amino acid dephosphorylation; exit from mitosis

Disease: Congenital Cataracts, Facial Dysmorphism, And Neuropathy

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