Recombinant Protein wingless (wg); Protein wingless; Protein Wnt-1 Protein int-1 dInt-1 dWnt-1

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 2L; NT_033779.4 (7307161..7316255). Location: 27F1-27F1; 2-21.9 cM

E Coli or Yeast or Baculovirus or Mammalian Cell

>=90% (lot specific)

Liquid containing glycerol

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterile filter available upon request.

Low endotoxin available upon request.

Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of wg recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

51,986 Da[Similar Products]

wingless

CG4889 gene product from transcript CG4889-RA; CG4889-PA; CG4889-PB; Wnt-1; bristled; complementation group I; flag; glazed; spade; sternopleural; wg-PA; wg-PB; wingless

Protein wingless

WNTG_DROME

Function: Binds as a ligand to a family of frizzled seven-transmembrane receptors and acts through a cascade of genes on the nucleus. Segment polarity protein. May be a growth factor. Acts on neighboring cells to regulate at least one gene, the homeobox segmentation gene engrailed. Wg signal represses arm phosphorylation. Wg signaling operates by inactivating the sgg repression of engrailed autoactivation. Wg and Wnt2 have a role in the developing trachea and together are responsible for all dorsal trunk formation. Wg also acts in the developing epidermis. Acts as a morphogen, and diffuses long distances despite its lipidation. Lipophorin is required for diffusion, probably by acting as vehicle for its movement, explaining how it can spread over long distances despite its lipidation. In non-neuronal cells, wls directs wg secretion via clathrin-mediated endocytosis and the retromer complex (a conserved protein complex consisting of Vps26 and Vps35) to sustain a wls traffic loop encompassing the Golgi, the cell surface, an endocytic compartment and a retrograde route leading back to the Golgi. In neuronal cells (the larval motorneuron NMJ), wg signal moves across the synapse through the release of wls-containing exosome-like vesicles. Ref.11 Ref.15 Ref.16 Ref.17

Subunit structure: Monomer; folds by intramolecular disulfide bonds. Interacts with porcupine (por). Interacts with wls; in the Golgi. Ref.8 Ref.12 Ref.15

Subcellular location: Secreted. Cell junction ? synapse. Membrane; Lipid-anchor. Secreted ? extracellular space ? extracellular matrix. Note: Palmitoylation converts wg into a membrane-anchored protein that is partitioned into specialized lipid raft microdomains before secretion. Possibly associated with the extracellular matrix. Ref.3 Ref.13 Ref.15 Ref.17

Tissue specificity: Segmented expression in embryos. In embryonic tracheal cells, expression is in stripes flanking the tracheal placode. Ref.1 Ref.11

Developmental stage: Expressed throughout development, but barely detectable in *****s. Ref.1 Ref.2

Post-translational modification: Palmitoylated by porcupine, acylation is a prerequisite. The lipid group functions as a sorting signal, targeting the ligand to polarized vesicles that transport wg to unique sites at the cell surface. Major form is glycosylated at 2 sites, glycosylation is stimulated by porcupine at the ER, again acylation is a prerequisite. Ref.12

Disruption phenotype: Accumulation in the Golgi, thereby preventing secretion. Ref.16

Sequence similarities: Belongs to the Wnt family.

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