For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a

5A1-1422

Rat

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised

Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.

Small volumes of anti-ABCA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rat anti Mouse ABCA1 antibody, clone 5A1-1422 recognizes murine adenosine triphosphate (ATP) Binding cassette transporter 1 (ABCA1). The ABC transporters are a large family of conserved proteins that transport a wide variety of molecules across cellular membranes. ABCA1 is a member of the ABC-A sub-family, which acts as a lipid translocator. The molecule was originally identified as a scavenger receptor on macrophages and research shows that ABCA1 also plays a major role in cholesterol metabolism. ABCA1 may play an important role in protecting against cardiovascular disease. Mutations in ABCA1 gene have been associated with Tangiers disease, a genetic disorder of lipid metabolism, and familial high density lipoprotein (HDL) deficiency.

Flow cytometry (FC/FACS)

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat

Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA1:RPE

Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA1:Alexa Fluor 488 (MBS219757A488)

Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA1:FITC (MBS219647)

253,912 Da

ATP-binding cassette, sub-family A (ABC1), member 1

ATP-binding cassette sub-family A member 1; ATP-binding cassette 1; ATP-binding cassette transporter 1

ATP-binding cassette sub-family A member 1

Abc1; ABC-1; ATP-binding cassette 1??[Similar Products]

ABCA1_MOUSE

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. In humans, this protein functions as a cholesterol efflux pump in the cellular lipid removal pathway. Mutations in the human gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq, Jul 2008]

ABCA1: an ATP-binding cassette (ABC) transporter that regulates cholesterol homeostasis and insulin secretion in beta-cells, and protects against cardiovascular disease. Inactivation of ABCA1 in beta-cells markedly impairs glucose tolerance and insulin secretion in mice. Rosiglitazone, an activator of the peroxisome proliferator-activated receptor-gamma, upregulates ABCA1 in beta-cells. ABCA1 expression in beta-cells is required for the beneficial effects of rosiglitazone on glucose tolerance. Mediates the transport of cholesterol and phospholipids from cells to HDL apolipoproteins, modulating HDL levels and atherogenesis. Induced by bacterial lipopolysaccharides (LPS) and repressed by ZNF202. Phosphorylation on Ser-2054 regulates phospholipid efflux. Unsaturated fatty acids destabilize ABCA1 by activating a PKC-delta pathway that phosphorylates ABCA1 serines. Defects in ABCA1 are a cause of high density lipoprotein deficiencies.

Protein type: Membrane protein, integral; Transporter, ABC family; Membrane protein, multi-pass; Vesicle; Transporter

Cellular Component: Golgi apparatus; cell surface; intracellular membrane-bound organelle; membrane; integral to plasma membrane; endocytic vesicle; perinuclear region of cytoplasm; integral to membrane; plasma membrane; phagocytic vesicle; lipid raft; external side of plasma membrane

Molecular Function: apolipoprotein A-I receptor activity; transporter activity; ATPase activity; nucleotide binding; apolipoprotein binding; ATPase binding; small GTPase binding; apolipoprotein A-I binding; protein binding; syntaxin binding; phospholipid transporter activity; ATPase activity, coupled to transmembrane movement of substances; cholesterol transporter activity; anion transmembrane transporter activity; ATP binding; receptor binding

Biological Process: endosome transport; platelet dense granule organization and biogenesis; phospholipid efflux; transport; positive regulation of cAMP biosynthetic process; protein amino acid lipidation; interleukin-1 beta secretion; transmembrane transport; phospholipid homeostasis; peptide secretion; cholesterol metabolic process; response to high density lipoprotein stimulus; regulation of Cdc42 protein signal transduction; cholesterol transport; intracellular cholesterol transport; cholesterol efflux; lipoprotein metabolic process; phospholipid translocation; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; reverse cholesterol transport; lipoprotein biosynthetic process; lysosome organization and biogenesis; phospholipid transport; phagocytosis, engulfment

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