CD230 antibody (ROS-BH1)

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

ROS-BH1

Mouse

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Liquid; Phosphate Buffered Saline

1.0 mg/ml (lot specific)

Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.

Small volumes of anti-CD230 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mouse anti Sheep CD230 (Prpsc) antibody, clone ROS-BH1 recognizes the prion disease form of prion protein known as CD230. Transmissible spongiform encephalopathies (TSEs) or prion diseases are fatal infectious neurodegenerative diseases of humans and animals, and known as Scrapie (sheep and goats), BSE (cattle), CWD (cervine/deer) and CJD (humans). These diseases are biologically unique, as they are believed by some to be transmitted by an infectious agent comprised only of protein, with no nucleic acid component. Clinically, these diseases present with motor disturbances and behavioral changes. The major pathological changes seen are neuronal loss, vacuolation (spongiform change), proliferation and branching of glial cells, astrocytic proliferation and accumulation of the prion protein PrPSc, which can form amyloid plaques.
CD230. Also known as the prion protein (PrP) exists in two alternate forms; a normal cellular form (PrPc) and a disease-associated form (PrPSc).The normal and pathological forms of the prion protein have identical amino acid sequences and differ only in their folded tertiary structure and biochemical properties.

Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Flow Cytometry analysis of ovine PBMC's using Mouse anti Sheep CD230, clone ROS-BH1 . Images courtesy of The Roslin Institute

Western Blot analysis of various tissues using Mouse anti Sheep CD230, clone ROS-BH1 . Lane 1 PRPC uninfected ovine tissue, Lane 2 PRPSc Lane 3 PrPSc ovine CH1641 scrapie, Lane 4 PrPSc natural scrapie, Lane 5 PrPSc caprine BSE, Lane 6 PrPSc bovine BSE. Image courtesy of the Roslin Institute.

Flow Cytometry analysis of CD230. Ovine PBMC's stained using Mouse anti Sheep CD230, clone ROS-BH1 as the primary antibody followed by anti mouse IgG conjugated to APC. Gated on sytox blue negative live cells and the PBMC region determined by FSC v. SSC profile. Image courtesy of The Roslin Institute.

major prion protein

Major prion protein

PRP; SIP; PrP??[Similar Products]

PRIO_SHEEP

Its primary physiological function is unclear. Has cytoprotective activity against internal or environmental stresses. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu2+ or ZN2+ for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains ().

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