Product Name
ANTXR2, siRNA
Full Product Name
ANTXR2 siRNA (Mouse)
Product Synonym Names
Anthrax toxin receptor 2
Product Gene Name
ANTXR2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q6DFX2
Specificity
ANTXR2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ANTXR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ANTXR2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ANTXR2 sirna
siRNA to inhibit ANTXR2 expression using RNA interference
Applications Tested/Suitable for ANTXR2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ANTXR2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_598499.1
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NCBI GenBank Nucleotide #
NM_133738.1
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UniProt Primary Accession #
Q6DFX2
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UniProt Secondary Accession #
Q99L17[Other Products]
UniProt Related Accession #
Q6DFX2[Other Products]
Molecular Weight
53,184 Da
NCBI Official Full Name
anthrax toxin receptor 2
NCBI Official Synonym Full Names
anthrax toxin receptor 2
NCBI Official Symbol
Antxr2??[Similar Products]
NCBI Official Synonym Symbols
CMG2; CMG-2; cI-35; AW561899; 2310046B19Rik
??[Similar Products]
NCBI Protein Information
anthrax toxin receptor 2
UniProt Protein Name
Anthrax toxin receptor 2
Protein Family
Anthrax toxin receptor
UniProt Gene Name
Antxr2??[Similar Products]
UniProt Entry Name
ANTR2_MOUSE
UniProt Comments for ANTXR2
ANTXR2: Necessary for cellular interactions with laminin and the extracellular matrix. Defects in ANTXR2 are the cause of infantile systemic hyalinosis (ISH). This autosomal recessive syndrome is similar to JHF, but has an earlier onset and a more severe course. Symptoms appear at birth or within the first months of life, with painful, swollen joint contractures, osteopenia, osteoporosis and livid red hyperpigmentation over bony prominences. Patients develop multiple subcutaneous skin tumors and gingival hypertrophy. Hyaline deposits in multiple organs, recurrent infections and intractable diarrhea often lead to death within the first 2 years of life. Surviving children may suffer from severely reduced mobility due to joint contractures. Defects in ANTXR2 are the cause of juvenile hyaline fibromatosis (JHF). JHF is an autosomal recessive syndrome that is similar to ISH but takes a milder course. It is characterized by hyaline deposition in the dermis, multiple subcutaneous skin tumors and gingival hypertrophy, followed by progressive joint contractions, osteopenia and osteoporosis that may lead to a severe limitation of mobility. Belongs to the ATR family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, misc.; Membrane protein, integral
Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane
Molecular Function: metal ion binding; receptor activity
Biological Process: reproductive process
Research Articles on ANTXR2
1. blood pressure measured under anesthesia in Antxr2 siRNA-injected mice rose significantly compared with that of the controls, suggesting that ANTXR2 is a causative gene in the human 4q21 GWAS-blood pressure locus.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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