For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

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Small volumes of ABCA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2603265 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ATP Binding Cassette Transporter A1 (ABCA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ABCA1. The ELISA analytical biochemical technique of the MBS2603265 kit is based on ABCA1 antibody-ABCA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ABCA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ABCA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

254,302 Da

ATP binding cassette subfamily A member 1

ATP-binding cassette sub-family A member 1

ATP-binding cassette sub-family A member 1

ABC1; CERP; ABC-1; ATP-binding cassette 1??[Similar Products]

ABCA1_HUMAN

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq, Jul 2008]

ABCA1: an ATP-binding cassette (ABC) transporter that regulates cholesterol homeostasis and insulin secretion in beta-cells, and protects against cardiovascular disease. Inactivation of ABCA1 in beta-cells markedly impairs glucose tolerance and insulin secretion in mice. Rosiglitazone, an activator of the peroxisome proliferator-activated receptor-gamma, upregulates ABCA1 in beta-cells. ABCA1 expression in beta-cells is required for the beneficial effects of rosiglitazone on glucose tolerance. Mediates the transport of cholesterol and phospholipids from cells to HDL apolipoproteins, modulating HDL levels and atherogenesis. Induced by bacterial lipopolysaccharides (LPS) and repressed by ZNF202. Phosphorylation on Ser-2054 regulates phospholipid efflux. Unsaturated fatty acids destabilize ABCA1 by activating a PKC-delta pathway that phosphorylates ABCA1 serines. Defects in ABCA1 are a cause of high density lipoprotein deficiencies.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, ABC family; Vesicle

Chromosomal Location of Human Ortholog: 9q31.1

Cellular Component: endocytic vesicle; external side of plasma membrane; Golgi apparatus; integral to plasma membrane; lipid raft; perinuclear region of cytoplasm; phagocytic vesicle; plasma membrane

Molecular Function: anion transmembrane transporter activity; apolipoprotein A-I binding; apolipoprotein A-I receptor activity; apolipoprotein binding; ATP binding; ATPase activity, coupled to transmembrane movement of substances; ATPase binding; cholesterol binding; cholesterol transporter activity; phospholipid binding; phospholipid transporter activity; protein binding; receptor binding; small GTPase binding; syntaxin binding

Biological Process: cellular lipid metabolic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; endosome transport; G-protein coupled receptor protein signaling pathway; interleukin-1 beta secretion; intracellular cholesterol transport; lipoprotein metabolic process; lysosome organization and biogenesis; peptide secretion; phagocytosis, engulfment; phospholipid efflux; phospholipid homeostasis; phospholipid translocation; platelet dense granule organization and biogenesis; positive regulation of cAMP biosynthetic process; protein amino acid lipidation; regulation of Cdc42 protein signal transduction; response to drug; response to low density lipoprotein stimulus; response to nutrient; reverse cholesterol transport

Disease: Hypercholesterolemia, Familial; Hypoalphalipoproteinemia, Primary; Tangier Disease

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