Anti -Csk Binding Protein (CBP, PAG, Phosphoprotein Associated with GEMs)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 15; NC_000015.9 (75074425..75095539). Location: 15q24.1

Polyclonal

IgG

Rabbit

Recognizes human PAG/Cbp. Species Crossreactivity: mouse.

Highly Purified
95% SDS-PAGE

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-CSK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Csk binding protein, also known as PAG (phosphoprotein associated with GEMs), is a ubiquitously expressed 46kD transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80kD molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.

Antibodies; Abs to Binding Proteins

Western Blot (WB), Immunoprecipitation (IP)

Suitable for use in Immunoprecipitation and Western Blot.,

50,704 Da[Similar Products]

c-src tyrosine kinase

tyrosine-protein kinase CSK; C-Src kinase; OTTHUMP00000183471; protein-tyrosine kinase CYL

Tyrosine-protein kinase CSK

CSK_HUMAN

CSK: a tyrosine kinase of the Csk family that phosphorylates and inhibits Src family kinases. Specifically phosphorylates Y504 on LCK, which acts as a negative regulatory site. Csk and the phospho-tyrosine phosphatase CD45 reciprocally regulate phosphorylation of the inhibitory tyrosine of the Src family kinases Lck and Fyn during T-cell receptor mediated signaling during thymic development.

Protein type: Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Kinase, protein; EC 2.7.10.2; TK group; Csk family

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; intercellular junction; cytosol; lipid raft

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein phosphatase binding; ATP binding; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of kinase activity; negative regulation of phagocytosis; platelet activation; cell migration; central nervous system development; negative regulation of Golgi to plasma membrane protein transport; T cell receptor signaling pathway; protein amino acid phosphorylation; regulation of cell proliferation; regulation of cytokine production; negative regulation of cell proliferation; positive regulation of MAP kinase activity; T cell costimulation; negative regulation of interleukin-6 production; morphogenesis of an epithelium; innate immune response; oligodendrocyte differentiation; brain development; cell differentiation; blood coagulation; negative regulation of bone resorption; transmembrane receptor protein tyrosine kinase signaling pathway

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