HBGF3; INT2; Heparin-binding growth factor 3; Murine Mammary Tumor Virus Integration Site(v-int-2)Oncogene Homolog; INT-2 Proto-Oncogene Protein; Proto-oncogene Int-2

For Research Use Only. Not for use in diagnostic procedures.

> 90%

Freeze-Dried Powder; 100mMNaHCO3, 500mMNaCl, pH8.3, Containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Refer to the datasheet for activity details. For other activity applications, researchers should evaluate this protein for suitability in their model system.

Small volumes of FGF3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Cell?Culture,?Activity?Assays

Fibroblast Growth Factor 3 (FGF3) also known as INT-2 proto-oncogene protein is a member of the fibroblast growth factor family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Besides, Fibroblast Growth Factor Receptor 2 (FGFR2) has been identified as an interactor of FGF3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF3 and recombinant human FGFR2. Briefly, FGF3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to FGFR2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-Fibroblast Growth Factor Receptor 2 (FGFR2) pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of FGF3 and FGFR2 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of FGF3 with FGFR2.

fibroblast growth factor 3

fibroblast growth factor 3

Fibroblast growth factor 3

INT2; FGF-3; HBGF-3??[Similar Products]

FGF3_HUMAN

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its similarity with mouse fgf3/int-2, a proto-oncogene activated in virally induced mammary tumors in the mouse. Frequent amplification of this gene has been found in human tumors, which may be important for neoplastic transformation and tumor progression. Studies of the similar genes in mouse and chicken suggested the role in inner ear formation. [provided by RefSeq, Jul 2008]

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