MGC22233; LPS-binding protein; lipopolysaccharide-binding protein

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Rabbit LBP. No significant cross-reactivity or interference between Rabbit LBP and analogues was observed.

Store at 2-8 degree C.

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Small volumes of LBP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS287563 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lipopolysaccharide-binding protein (LBP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LBP. The ELISA analytical biochemical technique of the MBS287563 kit is based on LBP antibody-LBP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LBP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LBP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Description: This assay employs a two-site sandwich ELISA to quantitate LBP in samples. An antibody specific for LBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: LPS-binding protein (LBP) is serum factor known to regulate the endotoxin-induced cellular immune response. Sepsis is a morbid condition induced by a toxin, the introduction or accumulation of which is most commonly caused by infection or trauma. Sepsis-inducing toxins have been found associated with pathogenic bacteria, viruses, plants and venoms.
Among the well described bacterial toxins are the endotoxins or lipopolysaccharides (LPS) of the gram-negative bacteria. Upon introduction of LPS into the blood it binds to lipopolysaccharide binding protein (LBP). LBP recognizes the lipid A region of LPS and forms high affinity complexes with both rough and smooth form LPS. During the acute phase, LBP is synthesized by hepatocytes, and reaches very high concentrations in serum.

54,001 Da

lipopolysaccharide-binding protein

Lipopolysaccharide-binding protein

LBP??[Similar Products]

Plays a role in the innate immune response (PubMed:2402637). Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria (PubMed:2427635). Acts as an affinity enhancer for CD14, facilitating its association with LPS (). Promotes the release of cytokines in response to bacterial lipopolysaccharide (PubMed:2402637).

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