CHD7; CRG; HH5; IS3; KAL5; KIAA1416; Chromodomain-helicase-DNA-binding protein 7; ATP-dependent helicase CHD7; CHD-7; chromodomain helicase DNA binding protein 7

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Multiple isoforms of CHD7 are known to exist.

CHD7 Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL (lot specific)

CHD7 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Small volumes of anti-CHD7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

CHD7 Antibody: CHD proteins belong to a superfamily of proteins of ATP-dependent chromatin remodeling enzymes that have a unique combination of functional domains, including two N-terminal chromodomains, a SNF2-like ATPase/helicase domain and a DNA-binding domain. These proteins are thought to play a role in early embryonic development by affecting chromatin structure and gene expression. Mutations in one member of this family, CHD7, result in CHARGE syndrome. It colocalizes with embryonic stem (ES) cell master regulators OCT4/POU5F1, SOX2, and NANOG and is thought to modulate ES-specific gene transcription. Together with SOX2, CHD7 has been suggested to also regulate several human disease genes.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

CHD7 antibody can be used for detection of CHD7 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western blot analysis of CHD7 in SK-N-SH cell lysate with CHD7 antibody at (A) 1 and (B) 2 μg/mL.

Immunohistochemistry of CHD7 in mouse brain tissue with CHD7 antibody at 5 μg/mL.

Immunofluorescence of CHD7 in mouse brain tissue with CHD7 antibody at 20 μg/mL.

101,085 Da

chromodomain helicase DNA binding protein 7

chromodomain-helicase-DNA-binding protein 7; CHARGE association; ATP-dependent helicase CHD7; chromodomain helicase DNA binding protein 7 isoform CRA_e

Chromodomain-helicase-DNA-binding protein 7

KIAA1416; CHD-7??[Similar Products]

CHD7_HUMAN

This gene encodes a protein that contains several helicase family domains. Mutations in this gene have been found in some patients with the CHARGE syndrome. [provided by RefSeq, Jul 2008]

CHD-7: Probable transcription regulator. May interact with CTCF. Interacts with CHD8. Widely expressed in fetal and ***** tissues. Belongs to the SNF2/RAD54 helicase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; DNA-binding; EC 3.6.4.12; Helicase

Chromosomal Location of Human Ortholog: 8q12.2

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; chromatin binding; helicase activity; ATP binding

Biological Process: limb development; heart morphogenesis; central nervous system development; olfactory behavior; blood circulation; positive regulation of multicellular organism growth; palate development; female genitalia development; regulation of growth hormone secretion; olfactory nerve development; embryonic hindlimb morphogenesis; ***** walking behavior; sensory perception of sound; regulation of neurogenesis; regulation of transcription, DNA-dependent; skeletal development; T cell differentiation; ***** heart development; inner ear morphogenesis; transcription, DNA-dependent; in utero embryonic development; olfactory bulb development; semicircular canal morphogenesis; cranial nerve development; chromatin modification; genitalia development; retina development in camera-type eye; artery morphogenesis; cognition; nose development; rRNA processing

Disease: Charge Syndrome; Tracheoesophageal Fistula With Or Without Esophageal Atresia; Hypogonadotropic Hypogonadism 5 With Or Without Anosmia

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