For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against ABCA1. It has beenselected for its ability to recognize ABCA1 in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Liquid

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Small volumes of anti-ABCA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Western Blot: Sample: Recombinant ALOX5, Human.

DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue

DAB staining on IHC-P; Samples: Human Liver Tissue.

DAB staining on IHC-P; Samples: Human Stomach Tissue.

DAB staining on IHC-P; Samples: Human Liver Cancer Tissue.

254,302 Da

ATP binding cassette subfamily A member 1

ATP-binding cassette sub-family A member 1

ATP-binding cassette sub-family A member 1

ABC1; CERP; ABC-1; ATP-binding cassette 1??[Similar Products]

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq, Jul 2008]

ABCA1: an ATP-binding cassette (ABC) transporter that regulates cholesterol homeostasis and insulin secretion in beta-cells, and protects against cardiovascular disease. Inactivation of ABCA1 in beta-cells markedly impairs glucose tolerance and insulin secretion in mice. Rosiglitazone, an activator of the peroxisome proliferator-activated receptor-gamma, upregulates ABCA1 in beta-cells. ABCA1 expression in beta-cells is required for the beneficial effects of rosiglitazone on glucose tolerance. Mediates the transport of cholesterol and phospholipids from cells to HDL apolipoproteins, modulating HDL levels and atherogenesis. Induced by bacterial lipopolysaccharides (LPS) and repressed by ZNF202. Phosphorylation on Ser-2054 regulates phospholipid efflux. Unsaturated fatty acids destabilize ABCA1 by activating a PKC-delta pathway that phosphorylates ABCA1 serines. Defects in ABCA1 are a cause of high density lipoprotein deficiencies.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ABC family; Vesicle

Chromosomal Location of Human Ortholog: 9q31.1

Cellular Component: endocytic vesicle; endoplasmic reticulum membrane; external side of plasma membrane; Golgi apparatus; high-density lipoprotein particle; integral component of plasma membrane; intracellular membrane-bound organelle; membrane raft; perinuclear region of cytoplasm; phagocytic vesicle; plasma membrane

Molecular Function: anion transmembrane transporter activity; apolipoprotein A-I binding; apolipoprotein A-I receptor activity; apolipoprotein binding; ATP binding; ATPase activity; ATPase activity, coupled to transmembrane movement of substances; ATPase binding; cholesterol binding; cholesterol transporter activity; phosphatidylcholine-translocating ATPase activity; phosphatidylserine-translocating ATPase activity; phospholipid binding; phospholipid transporter activity; protein binding; receptor binding; small GTPase binding; syntaxin binding

Biological Process: anion transmembrane transport; apolipoprotein A-I-mediated signaling pathway; cellular response to cholesterol; cellular response to retinoic acid; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; endosomal transport; G-protein coupled receptor signaling pathway; high-density lipoprotein particle assembly; interleukin-1 beta secretion; intracellular cholesterol transport; lysosome organization and biogenesis; negative regulation of cholesterol storage; negative regulation of macrophage derived foam cell differentiation; phagocytosis, engulfment; phospholipid efflux; phospholipid homeostasis; phospholipid translocation; platelet dense granule organization and biogenesis; positive regulation of cAMP biosynthetic process; positive regulation of cholesterol efflux; protein lipidation; regulation of Cdc42 protein signal transduction; regulation of high-density lipoprotein particle assembly; regulation of lipid metabolic process; response to drug; response to laminar fluid shear stress; response to low density lipoprotein stimulus; response to nutrient; reverse cholesterol transport

Disease: Hypercholesterolemia, Familial; Hypoalphalipoproteinemia, Primary; Tangier Disease

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