Product Name
IGBP1, siRNA
Full Product Name
IGBP1 siRNA (Mouse)
Product Synonym Names
PC52; Immunoglobulin-binding protein 1; Alpha4 phosphoprotein; CD79a-binding protein 1; Lymphocyte signal transduction molecule alpha 4; Protein phosphatase 2/4/6 regulatory subunit; p52
Product Gene Name
IGBP1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q61249
Specificity
IGBP1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse IGBP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of IGBP1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
IGBP1 sirna
siRNA to inhibit IGBP1 expression using RNA interference
Applications Tested/Suitable for IGBP1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for IGBP1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_032810.4
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NCBI GenBank Nucleotide #
NM_008784.3
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UniProt Primary Accession #
Q61249
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UniProt Related Accession #
Q61249[Other Products]
Molecular Weight
38,971 Da
NCBI Official Full Name
immunoglobulin-binding protein 1
NCBI Official Synonym Full Names
immunoglobulin (CD79A) binding protein 1
NCBI Official Symbol
Igbp1??[Similar Products]
NCBI Official Synonym Symbols
p52; Pc52; Tap42; C81413; AI115586; AI662457; AW208785
??[Similar Products]
NCBI Protein Information
immunoglobulin-binding protein 1
UniProt Protein Name
Immunoglobulin-binding protein 1
UniProt Synonym Protein Names
Alpha4 phosphoprotein; CD79a-binding protein 1; Lymphocyte signal transduction molecule alpha 4; Protein phosphatase 2/4/6 regulatory subunit; p52
Protein Family
Immunoglobulin-binding protein
UniProt Gene Name
Igbp1??[Similar Products]
UniProt Synonym Gene Names
Pc52??[Similar Products]
UniProt Entry Name
IGBP1_MOUSE
UniProt Comments for IGBP1
IGBP1: Associated to surface IgM-receptor; may be involved in signal transduction. May be involved in regulation of the catalytic activity of type 2A-related serine/threonine phosphatases. Associates with PP2A-alpha and PP2A-beta catalytic subunits, and with PP4 and PP6. Interacts with MID1 and MID2. Ubiquitously expressed with highest levels in heart, skeletal muscle and pancreas. Belongs to the IGBP1/TAP42 family.
Protein type: Phosphatase, regulatory subunit; Motility/polarity/chemotaxis
Cellular Component: microtubule; cytoplasm; cytosol
Molecular Function: protein domain specific binding; protein binding; protein phosphatase 2A binding; mitogen-activated protein kinase kinase binding; protein complex binding; protein phosphatase type 2A regulator activity
Biological Process: negative regulation of protein amino acid dephosphorylation; B cell activation; positive regulation of dephosphorylation; regulation of signal transduction; positive regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of stress-activated MAPK cascade
Research Articles on IGBP1
1. both the C-terminal Mid1-binding domain and the PP2Ac-binding determinants are required for Alpha4-mediated protection of PP2Ac from polyubiquitination and degradation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
