Mouse Cartilage oligomeric matrix protein (COMP) ELISA kit; EDM1; EPD1; MED; MGC131819; MGC149768; PSACH; THBS5; cartilage oligomeric matrix protein (pseudoachondroplasia; epiphyseal dysplasia 1; multiple) ; cartilage oligomeric matrix protein (pseudoachondropl; cartilage oligomeric matrix protein

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Mouse COMP. No significant cross-reactivity or interference between Mouse COMP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of COMP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS941188 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cartilage oligomeric matrix protein (COMP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COMP. The ELISA analytical biochemical technique of the MBS941188 kit is based on COMP antibody-COMP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COMP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COMP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Cartilage oligomeric matrix protein is a known biomarker for human cartilage turnover and mutations in human COMP result in heritable osteoarthritis cartilage dysplasias. Mouse models of the protein COMP mutations or in development as a diagnostic biomarker need a sensitive and specific COMP ELISA assay product. A very reliable and economical COMP ELISA kit is available in the United States for these applications as outlined in the products mo datasheet. Sold as complete kits, with primary antibody pre-bound to plate mo or wells, standard matrix protein COMP and secondary antibodies AMP or MBS detection reagents. No cross species - rat, guinea pig or human cartilage oligomeric protein - or cross reactions to related collagen type protein is seen by the matrix protein ELISA manufacturer. With ng/ml detection ranges, many typical research samples, such as cell lysates, tissue culture supernatants or mouse serum may be included in 1 kit assay formats.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COMP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COMP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COMP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COMP bound in the initial step. The color development is stopped and the intensity of the color is measured.

82,342 Da

cartilage oligomeric matrix protein

cartilage oligomeric matrix protein; thrombospondin-5

Cartilage oligomeric matrix protein

COMP??[Similar Products]

COMP_MOUSE

COMP: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7. Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1). EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COMP are the cause of pseudoachondroplasia (PSACH). PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood. Belongs to the thrombospondin family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: vitamin D binding; heparin binding; heparan sulfate proteoglycan binding; collagen binding; protein binding; protease binding; extracellular matrix structural constituent; fibronectin binding; calcium ion binding

Biological Process: limb development; extracellular matrix organization and biogenesis; apoptosis; cell adhesion; negative regulation of apoptosis

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