Full Product Name
ATRX siRNA (Human)
Product Synonym Names
RAD54L; XH2; Transcriptional regulator ATRX; ATP-dependent helicase ATRX; X-linked helicase II; X-linked nuclear protein; XNP; Znf-HX
Product Gene Name
ATRX sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P46100
Specificity
ATRX siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ATRX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ATRX sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ATRX sirna
siRNA to inhibit ATRX expression using RNA interference
Applications Tested/Suitable for ATRX sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ATRX. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000480.3
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NCBI GenBank Nucleotide #
NM_000489.4
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UniProt Primary Accession #
P46100
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UniProt Secondary Accession #
P51068; Q15886; Q59FB5; Q59H31; Q5H9A2; Q5JWI4; Q7Z2J1; Q9H0Z1; Q9NTS3; D3DTE2[Other Products]
UniProt Related Accession #
P46100[Other Products]
Molecular Weight
151,556 Da
NCBI Official Full Name
transcriptional regulator ATRX isoform 1
NCBI Official Synonym Full Names
alpha thalassemia/mental retardation syndrome X-linked
NCBI Official Symbol
ATRX??[Similar Products]
NCBI Official Synonym Symbols
JMS; SHS; XH2; XNP; ATR2; SFM1; MRX52; RAD54; MRXHF1; RAD54L; ZNF-HX
??[Similar Products]
NCBI Protein Information
transcriptional regulator ATRX
UniProt Protein Name
Transcriptional regulator ATRX
UniProt Synonym Protein Names
ATP-dependent helicase ATRX; X-linked helicase II; X-linked nuclear protein; XNP; Znf-HX
Protein Family
Transcriptional regulator
UniProt Gene Name
ATRX??[Similar Products]
UniProt Synonym Gene Names
RAD54L; XH2; XNP??[Similar Products]
UniProt Entry Name
ATRX_HUMAN
NCBI Summary for ATRX
The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Mutations in this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Aug 2013]
UniProt Comments for ATRX
ATRX: a global transcriptional regulator. Belongs to the SNF2 family of proteins, many of which modify gene expression via chromatin remodeling activity. Involved in the developmental silencing of imprinted genes in the brain. Contains one PxVxL motif, which is required for interaction with chromoshadow domains. This motif requires additional residues at -7, -6, +4 and +5 relative to the central V which contact the chromoshadow domain. Constitutive mutations in ATRX are associated with brain, facial, and genital abnormalities, and alpha thalassemia. Acquired mutations in ATRX have been observed in preleukemic conditions. Six alternatively spliced human isoforms have been described.
Protein type: Helicase; Ubiquitin conjugating system; DNA repair, damage; EC 3.6.4.12
Chromosomal Location of Human Ortholog: Xq21.1
Cellular Component: nucleoplasm; PML body; nuclear heterochromatin; nucleus
Molecular Function: protein binding; DNA helicase activity; DNA binding; zinc ion binding; histone binding; chromatin binding; helicase activity; DNA translocase activity; methylated histone residue binding; ATP binding
Biological Process: DNA damage response, signal transduction by p53 class mediator; transcription, DNA-dependent; positive regulation of telomere maintenance; DNA repair; Sertoli cell development; DNA duplex unwinding; replication fork processing; DNA recombination; chromatin remodeling; nucleosome assembly; regulation of transcription, DNA-dependent; DNA replication-independent nucleosome assembly; forebrain development; DNA methylation; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter
Disease: Mental Retardation-hypotonic Facies Syndrome, X-linked, 1; Alpha-thalassemia/mental Retardation Syndrome, X-linked; Alpha-thalassemia Myelodysplasia Syndrome
Research Articles on ATRX
1. negative nuclear staining in a subgroup of ileal neuroendocrine tumors
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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