Human ADAMTS-like protein 4 (ADAMTSL4) ELISA kit; TSRC1; OTTHUMP00000032789; thrombospondin repeat containing 1; ADAMTS-like 4

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human ADAMTSL4. No significant cross-reactivity or interference between Human ADAMTSL4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ADAMTSL4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS931950 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ADAMTS-like 4 (ADAMTSL4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAMTSL4. The ELISA analytical biochemical technique of the MBS931950 kit is based on ADAMTSL4 antibody-ADAMTSL4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAMTSL4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAMTSL4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAMTSL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTSL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTSL4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTSL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

116,545 Da

ADAMTS-like 4

ADAMTS-like protein 4; thrombospondin repeat-containing protein 1

ADAMTS-like protein 4

TSRC1; PP1396; UNQ2803/PRO34012; ADAMTSL-4??[Similar Products]

ATL4_HUMAN

This gene is a member of ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs)-like gene family and encodes a protein with seven thrombospondin type 1 repeats. The thrombospondin type 1 repeat domain is found in many proteins with diverse biological functions including cellular adhesion, angiogenesis, and patterning of the developing nervous system. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Sep 2014]

TSRC1: Positive regulation of apoptosis. May facilitate FBN1 microfibril biogenesis. Defects in ADAMTSL4 are a cause of autosomal recessive isolated ectopia lentis (ARI-EL). ARI-EL is a rare condition characterized by partial or complete displacement of the lens from its space resulting from defective zonule formation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: endoplasmic reticulum lumen

Molecular Function: protein binding; protease binding; metalloendopeptidase activity

Biological Process: protein amino acid O-linked glycosylation; extracellular matrix organization and biogenesis; cellular protein metabolic process; positive regulation of apoptosis; apoptosis; proteolysis; post-translational protein modification

Disease: Ectopia Lentis 2, Isolated, Autosomal Recessive; Ectopia Lentis Et Pupillae

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