Full Product Name
DNM1 siRNA (Human)
Product Synonym Names
DNM; Dynamin-1
Product Gene Name
DNM1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q05193
Specificity
DNM1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DNM1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of DNM1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DNM1 sirna
siRNA to inhibit DNM1 expression using RNA interference
Applications Tested/Suitable for DNM1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for DNM1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001005336.1
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NCBI GenBank Nucleotide #
NM_001005336.2
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UniProt Primary Accession #
Q05193
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UniProt Secondary Accession #
Q5SYX0; Q5SYX2; Q6P3T6; Q86VD2; A6NLM6[Other Products]
UniProt Related Accession #
Q05193[Other Products]
Molecular Weight
95,895 Da
NCBI Official Full Name
dynamin-1 isoform 2
NCBI Official Synonym Full Names
dynamin 1
NCBI Official Symbol
DNM1??[Similar Products]
NCBI Official Synonym Symbols
DNM; EIEE31
??[Similar Products]
NCBI Protein Information
dynamin-1
UniProt Protein Name
Dynamin-1
UniProt Gene Name
DNM1??[Similar Products]
UniProt Synonym Gene Names
DNM??[Similar Products]
UniProt Entry Name
DYN1_HUMAN
NCBI Summary for DNM1
This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Comments for DNM1
DYN1: a microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Concentrates within the presynaptic compartment and may participate in specialized neuronal functions such as rapid synaptic vesicle recycling. Part of a protein network that controls nucleation of actin from membranes. Contains one PH domain.
Protein type: EC 3.6.5.5; Hydrolase; Vesicle; Motility/polarity/chemotaxis; Motor; Microtubule-binding
Chromosomal Location of Human Ortholog: 9q34
Cellular Component: microtubule; membrane coat; plasma membrane
Molecular Function: GTPase activity; protein C-terminus binding; identical protein binding; protein binding; GTP binding; protein complex binding; nitric-oxide synthase binding; protein kinase binding
Biological Process: axon guidance; receptor-mediated endocytosis; endosome organization and biogenesis; receptor internalization; ephrin receptor signaling pathway; endocytosis
Disease: Epileptic Encephalopathy, Early Infantile, 31
Research Articles on DNM1
1. Dynamin 1 and dynamin 2 activity are not essential for Chlamydia trachomatis internalization but is required for normal development.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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