Product Name
Gonadotropin Releasing Hormone (GnRH), ELISA Kit
Full Product Name
Gonadotropin Releasing Hormone (GnRH) ELISA Kit
Product Synonym Names
GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1
Product Gene Name
GnRH elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
Request for Current Manual Insert
Request Current Manual
3D Structure
ModBase 3D Structure for P07490
Specificity
This assay has high sensitivity and excellent specificity for detection of Gonadotropin Releasing Hormone (GnRH).
No significant cross-reactivity or interference between Gonadotropin Releasing Hormone (GnRH) and analogues was observed.
Assay Type
Competitive Inhibition
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Detection Range
12.35-1000pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Gonadotropin Releasing Hormone (GnRH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Gonadotropin Releasing Hormone (GnRH) were tested on 3 different plates, 8 replicates in each plate.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GnRH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for GnRH purchase
MBS2087002 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Gonadotropin Releasing Hormone (GnRH) ELISA Kit target analytes in
biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GnRH. The ELISA analytical biochemical technique of the MBS2087002 kit is based on GnRH antibody-GnRH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GnRH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GnRH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GnRH elisa kit
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gonadotropin Releasing Hormone (GnRH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gonadotropin Releasing Hormone (GnRH) and unlabeled Gonadotropin Releasing Hormone (GnRH) (Standards or samples) with the pre-coated antibody specific to Gonadotropin Releasing Hormone (GnRH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gonadotropin Releasing Hormone (GnRH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gonadotropin Releasing Hormone (GnRH) in the sample.
Product Categories/Family for GnRH elisa kit
Endocrinology; Reproductive science; Neuroscience; Genetic science; Hormone metabolism
Typical Testing Data/Standard Curve (for reference only) of GnRH elisa kit
NCBI/Uniprot data below describe general gene information for GnRH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036899.1
[Other Products]
NCBI GenBank Nucleotide #
NM_012767.2
[Other Products]
UniProt Primary Accession #
P07490
[Other Products]
UniProt Related Accession #
P07490; P55248[Other Products]
Molecular Weight
10,500 Da
NCBI Official Full Name
progonadoliberin-1
NCBI Official Synonym Full Names
gonadotropin releasing hormone 1
NCBI Official Symbol
Gnrh1??[Similar Products]
NCBI Official Synonym Symbols
Gnrh; Lhrh; SH-4; Gnrha; Rgnrhg1
??[Similar Products]
NCBI Protein Information
progonadoliberin-1
UniProt Protein Name
Progonadoliberin-1
UniProt Synonym Protein Names
Progonadoliberin I
UniProt Gene Name
Gnrh1??[Similar Products]
UniProt Synonym Gene Names
Gnrh; GnRH-I; LH-RH I??[Similar Products]
NCBI Summary for GnRH
involved in induction of ovulation; may inhibit anterior pituitary cell apoptosis [RGD, Feb 2006]
UniProt Comments for GnRH
Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones.
Research Articles on GnRH
1. Under-nutrition delayed puberty onset and decreased hypothalamic GnRH expression.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.