Product Name
CLCF1, Recombinant Protein
Product Gene Name
CLCF1 recombinant protein
[Similar Products]
Matching Pairs
Antibody: NNT-1/BCSF-3 (MBS550313)
Immunogen: CLCF1 (MBS553713)
Matching Pairs
Antibody: NNT-1/BCSF-3 (MBS553554)
Immunogen: CLCF1 (MBS553713)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF
3D Structure
ModBase 3D Structure for Q9UBD9
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC.
Form/Format
Lyophilized; PBS, pH 7.2
Source Note
Optimized DNA sequence encoding Human CLCF1 mature chain was expressed in ECOLI. Recombinant Human Cardiotrophin-like cytokine factor 1 encodes amino acid residues 28-225.
Biological Activity
NT-1 weakly supports chick E8 DRG neurite outgrowth at a concentration of 1.0 ng/ml
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of CLCF1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for CLCF1 recombinant protein
SDS-PAGE
Application Notes for CLCF1 recombinant protein
Recombinant CLCF1 migrates as an approximately 22 kDa protein under reducing conditions in SDS-PAGE.
NCBI/Uniprot data below describe general gene information for CLCF1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_037378.1
[Other Products]
NCBI GenBank Nucleotide #
NM_013246.3
[Other Products]
UniProt Primary Accession #
Q9UBD9
[Other Products]
UniProt Secondary Accession #
Q6NZA4; B4DNT4[Other Products]
UniProt Related Accession #
Q9UBD9[Other Products]
Molecular Weight
24,087 Da
NCBI Official Full Name
cardiotrophin-like cytokine factor 1 isoform 1
NCBI Official Synonym Full Names
cardiotrophin like cytokine factor 1
NCBI Official Symbol
CLCF1??[Similar Products]
NCBI Official Synonym Symbols
CLC; NR6; BSF3; NNT1; BSF-3; CISS2; NNT-1
??[Similar Products]
NCBI Protein Information
cardiotrophin-like cytokine factor 1
UniProt Protein Name
Cardiotrophin-like cytokine factor 1
UniProt Synonym Protein Names
B-cell-stimulating factor 3; BSF-3; Novel neurotrophin-1; NNT-1
Protein Family
Cardiotrophin-like cytokine factor
UniProt Gene Name
CLCF1??[Similar Products]
UniProt Synonym Gene Names
BSF3; CLC; NNT1; BSF-3; NNT-1??[Similar Products]
NCBI Summary for CLCF1
This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]
UniProt Comments for CLCF1
Cytokine with B-cell stimulating capability. Binds to and activates the ILST/gp130 receptor.
Research Articles on CLCF1
1. CLF-1, based on its for CLC and on two additional and independent sites for CNTFRalpha and sorLA, is a key player in CLC and CNTFRalpha signaling and turnover.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
