Product Name
PCDH15, Polyclonal Antibody
Full Product Name
PCDH15 Polyclonal Antibody
Product Gene Name
anti-PCDH15 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q96QU1
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity purification
Immunogen
Recombinant protein of human PCDH15
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-PCDH15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-PCDH15 antibody
Polyclonal
Applications Tested/Suitable for anti-PCDH15 antibody
Western Blot (WB)
Application Notes for anti-PCDH15 antibody
WB: 1:500 - 1:2000
Western Blot (WB) of anti-PCDH15 antibody
Western blot analysis of extracts of various cell lines, using PCDH15 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
NCBI/Uniprot data below describe general gene information for PCDH15. It may not necessarily be applicable to this product.
NCBI Accession #
NP_149045.3
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NCBI GenBank Nucleotide #
NM_033056.3
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UniProt Primary Accession #
Q96QU1
[Other Products]
UniProt Secondary Accession #
Q5VY38; Q5VY39; Q6TRH8; Q8NDB9; Q96QT8; A6NL19; C6ZEF5; C6ZEF6; C6ZEF7[Other Products]
UniProt Related Accession #
Q96QU1[Other Products]
Molecular Weight
Calculated MW: 216kDa
Molecular Weight: 1955 (1962)
NCBI Official Full Name
protocadherin-15 isoform CD1-4
NCBI Official Synonym Full Names
protocadherin-related 15
NCBI Official Symbol
PCDH15??[Similar Products]
NCBI Official Synonym Symbols
USH1F; CDHR15; DFNB23
??[Similar Products]
NCBI Protein Information
protocadherin-15
UniProt Protein Name
Protocadherin-15
Protein Family
Protocadherin
UniProt Gene Name
PCDH15??[Similar Products]
UniProt Synonym Gene Names
USH1F??[Similar Products]
UniProt Entry Name
PCD15_HUMAN
NCBI Summary for PCDH15
This gene is a member of the cadherin superfamily. Family members encode integral membrane proteins that mediate calcium-dependent cell-cell adhesion. It plays an essential role in maintenance of normal retinal and cochlear function. Mutations in this gene result in hearing loss and Usher Syndrome Type IF (USH1F). Extensive alternative splicing resulting in multiple isoforms has been observed in the mouse ortholog. Similar alternatively spliced transcripts are inferred to occur in human, and additional variants are likely to occur. [provided by RefSeq, Dec 2008]
UniProt Comments for PCDH15
Calcium-dependent cell-adhesion protein. Essential for maintenance of normal retinal and cochlear function.
Research Articles on PCDH15
1. The novel homozygous mutation in a family segregating non-syndromic hearing loss family supports previous reported observations that PCDH15 does not only causes Usher syndrome type 1F, but also DFNB23.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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