Product Name
Circadian locomoter output cycles protein kaput (Clk), Recombinant Protein
Full Product Name
Recombinant Drosophila melanogaster Circadian locomoter output cycles protein kaput (Clk), partial
Product Gene Name
Clk recombinant protein
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O61735
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Species
Drosophila melanogaster (Fruit fly)
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Clk recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for Clk. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001014574.1
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NCBI GenBank Nucleotide #
NM_001014574.2
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UniProt Primary Accession #
O61735
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UniProt Secondary Accession #
O76342; O77137; Q59E25; Q9VSB0; A4V1L9; A4V1M0[Other Products]
UniProt Related Accession #
O61735[Other Products]
Molecular Weight
108,552 Da
NCBI Official Full Name
clock, isoform F
NCBI Official Synonym Full Names
Clock
NCBI Official Symbol
Clk??[Similar Products]
NCBI Official Synonym Symbols
bHLHe10; CG7391; clk; CLK; clock; CLOCK; dClck; dclk; dClk; dCLK; dCLK/JRK; dClock; dCLOCK; DmelCG7391; Jerk; jrk; Jrk; PAS1
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NCBI Protein Information
CG7391 gene product from transcript CG7391-RA
UniProt Protein Name
Circadian locomoter output cycles protein kaput
UniProt Synonym Protein Names
dCLOCK; dPAS1
UniProt Gene Name
Clk??[Similar Products]
UniProt Synonym Gene Names
CLOCK; jrk; PAS1??[Similar Products]
UniProt Comments for Clk
Circadian regulator that acts as a transcription factor and generates a rhythmic output with a period of about 24 hours. Oscillates in antiphase to the cycling observed for period (PER) and timeless (TIM). According to PubMed:9742131, reaches peak abundance within several hours of the dark-light transition at ZT0 (zeitgeber 0), whereas PubMed:9616122 describes bimodal oscillating expression with maximum at ZT5 and ZT23. Clock-cycle heterodimers activate cycling transcription of PER and TIM by binding to the E-box (5'-CACGTG-3') present in their promoters. Once induced, Period and Timeless block Clock's ability to transactivate their promoters.
Research Articles on Clk
1. propose that the dCLK/CYC-controlled TTFL operates differently in subsets of pacemaker neurons, which may contribute to their specific functions
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
