Product Name
14-3-3 alpha/beta, Monoclonal Antibody
Full Product Name
14-3-3 alpha/beta antibody
Product Synonym Names
Monoclonal 14-3-3 alpha/beta; Anti-14-3-3 alpha/beta; KCIP-1; KCIP1; YWHAB; HS1; GW128; YWHAA; KCIP 1; 14-3-3 alpha+beta; 14-3-3 alpha beta
Product Gene Name
anti-14-3-3 alpha/beta antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Form/Format
Supplied in citrate-tris-HCl buffer, pH 7.0 with 0.02% Proclin 300
Concentration
1 mg/ml (lot specific)
Biological Significance
14-3-3 alpha/beta prevents apoptosis by sequestering Bad to the cytosol.
Immunogen
14-3-3 alpha/beta antibody was raised in Mouse using a synthetic peptide (KLH-coupled) corresponding to near C-terminal residues of 14-3-3b/a protein as the immunogen
Preparation and Storage
Aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze/thaw cycles
Other Notes
Small volumes of anti-14-3-3 alpha/beta antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-14-3-3 alpha/beta antibody
Mouse monoclonal 14-3-3 alpha/beta antibody
Product Categories/Family for anti-14-3-3 alpha/beta antibody
Cell Cycle & Cell Death
Applications Tested/Suitable for anti-14-3-3 alpha/beta antibody
Western Blot (WB)
Application Notes for anti-14-3-3 alpha/beta antibody
WB: 1:1000-1:2000
Western Blot (WB) of anti-14-3-3 alpha/beta antibody
Western blot analysis on mouse liver lysates using anti-14-3-3 b/a monoclonal antibody.
NCBI/Uniprot data below describe general gene information for 14-3-3 alpha/beta. It may not necessarily be applicable to this product.
NCBI Accession #
NP_647539.1
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NCBI GenBank Nucleotide #
NM_139323.3
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UniProt Secondary Accession #
A8K9K2; E1P616[Other Products]
UniProt Related Accession #
P31946[Other Products]
Molecular Weight
27,850 Da
NCBI Official Full Name
14-3-3 protein beta/alpha
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta
NCBI Official Symbol
YWHAB??[Similar Products]
NCBI Official Synonym Symbols
HS1; GW128; YWHAA; KCIP-1; HEL-S-1
??[Similar Products]
NCBI Protein Information
14-3-3 protein beta/alpha
UniProt Protein Name
14-3-3 protein beta/alpha
UniProt Synonym Protein Names
Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1
UniProt Gene Name
YWHAB??[Similar Products]
UniProt Synonym Gene Names
KCIP-1??[Similar Products]
UniProt Entry Name
1433B_HUMAN
NCBI Summary for 14-3-3 alpha/beta
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for 14-3-3 alpha/beta
14-3-3 beta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 20q13.1
Cellular Component: focal adhesion; cytoplasmic vesicle membrane; transcriptional repressor complex; membrane; perinuclear region of cytoplasm; cytoplasm; melanosome; cytosol; nucleus
Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; enzyme binding; histone deacetylase binding; protein complex binding; phosphoprotein binding; transcription corepressor activity; phosphoserine binding
Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of catalytic activity; negative regulation of protein amino acid dephosphorylation; axon guidance; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; activation of MAPKK activity; apoptosis; protein heterooligomerization; MAPKKK cascade; small GTPase mediated signal transduction; Ras protein signal transduction; cytoplasmic sequestering of protein; insulin receptor signaling pathway; innate immune response; gene expression; vascular endothelial growth factor receptor signaling pathway; negative regulation of transcription, DNA-dependent; protein targeting
Research Articles on 14-3-3 alpha/beta
1. miR-152 controls both the expression of 14-3-3beta and HLA-G and exerts a dual role in tumor cells by both altering the immunogenicity and the tumorigenicity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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