Product Name
14-3-3 beta, Monoclonal Antibody
Full Product Name
14-3-3 beta antibody
Product Synonym Names
Monoclonal 14-3-3 beta; Anti-14-3-3 beta; YWHAB; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; beta polypeptide; 14-3-3 alpha; 14-3-3beta; 14.3.3 beta; 14-3-3 protein beta; 14-3-3 protein beta/alpha; 14-3-3 protein beta-subtype; 3-monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide; BOS_13300; brain protein 14-3-3; beta isoform; gw128; HS1; kcip-1; prepronerve growth factor RNH-1; protein 1054; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; RCJMB04_5d11; RP1-148E22.1; RP23-321M14.1; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma; TTpA010k20.1; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein; tyrosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein; alpha polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein; YWHAA
Product Gene Name
anti-14-3-3 beta antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P31946
Species Reactivity
Human, Mouse, Rat
Purity/Purification
14-3-3 beta antibody was purified by ammonium sulfate precipitation
Form/Format
Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol
Biological Significance
14-3-3, a family of acidic and soluble proteins, highly conserved in amino acid sequences from yeast to mammals, is expressed in all eukaryotic cells. Seven isoforms (beta, gamma, epsilon, eta, zeta, omega and t/?) encoded by seven distinct genes are identified in mammals and forms homo- and hetero- dimeric cup-shaped structures. As 14-3-3 is interacted with more than 100 binding partners, it regulates key proteins involved in various
biological processes such as signal trans-duction, cell cycle, transcriptional control, cell proliferation, apoptosis, and ion channel physiology. Most 14-3-3 requires phosphorylation of serine or threonine residues in the target sequence. This protein is abundantly expressed in the brain and has been detected in the cerebrospinal fluid of patients with different neurological disorders.
Immunogen
14-3-3 beta antibody was raised in Mouse using a recombinant human protein purified from E Coli as the immunogen
Preparation and Storage
Aliquot and store for 1 year at -20 degree C, avoid freeze/thaw cycles
Other Notes
Small volumes of anti-14-3-3 beta antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-14-3-3 beta antibody
Mouse monoclonal 14-3-3 beta antibody
Product Categories/Family for anti-14-3-3 beta antibody
Neuroscience
Applications Tested/Suitable for anti-14-3-3 beta antibody
Western Blot (WB)
Application Notes for anti-14-3-3 beta antibody
WB: 1:2000
Western Blot (WB) of anti-14-3-3 beta antibody
NCBI/Uniprot data below describe general gene information for 14-3-3 beta. It may not necessarily be applicable to this product.
NCBI Accession #
NP_647539.1
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NCBI GenBank Nucleotide #
NM_139323.3
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UniProt Primary Accession #
P31946
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UniProt Secondary Accession #
A8K9K2; E1P616[Other Products]
UniProt Related Accession #
P31946[Other Products]
Molecular Weight
27,850 Da
NCBI Official Full Name
14-3-3 protein beta/alpha
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta
NCBI Official Symbol
YWHAB??[Similar Products]
NCBI Official Synonym Symbols
HS1; GW128; YWHAA; KCIP-1; HEL-S-1
??[Similar Products]
NCBI Protein Information
14-3-3 protein beta/alpha
UniProt Protein Name
14-3-3 protein beta/alpha
UniProt Synonym Protein Names
Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1
UniProt Gene Name
YWHAB??[Similar Products]
UniProt Synonym Gene Names
KCIP-1??[Similar Products]
UniProt Entry Name
1433B_HUMAN
NCBI Summary for 14-3-3 beta
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for 14-3-3 beta
14-3-3 beta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 20q13.1
Cellular Component: focal adhesion; cytoplasmic vesicle membrane; transcriptional repressor complex; membrane; perinuclear region of cytoplasm; cytoplasm; melanosome; nucleus; cytosol
Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; enzyme binding; histone deacetylase binding; protein complex binding; phosphoprotein binding; transcription corepressor activity; phosphoserine binding
Biological Process: positive regulation of catalytic activity; epidermal growth factor receptor signaling pathway; negative regulation of protein amino acid dephosphorylation; axon guidance; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; activation of MAPKK activity; apoptosis; protein heterooligomerization; MAPKKK cascade; small GTPase mediated signal transduction; Ras protein signal transduction; cytoplasmic sequestering of protein; insulin receptor signaling pathway; innate immune response; gene expression; vascular endothelial growth factor receptor signaling pathway; negative regulation of transcription, DNA-dependent; protein targeting
Research Articles on 14-3-3 beta
1. miR-152 controls both the expression of 14-3-3beta and HLA-G and exerts a dual role in tumor cells by both altering the immunogenicity and the tumorigenicity
Precautions
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Disclaimer
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