Tyrosine 3-monocxygenase/tryptopan 5-monooxygenase activation protein beta polypeptide; Tyrosine 3-monocxygenase/tryptopan 5-monooxygenase activation protein; beta polypeptide; 14-3-3 beta; YWHAB; GW128; HS1; KCIP-1; Tyrosine 3-monocxygenase/tryptopan 5-monooxygenase activation protein; beta polypeptide 14 3 3 protein beta/alpha; Brain protein 14 3 3 beta isoform; GW 128; HS 1; KCIP 1; KCIP1; Protein 1054; Protein kinase C inhibitor protein 1; Tyrosine 3 Monooxygenase/Tryptophan 5 Monooxygenase Activation Protein Beta Polypeptide.

For Research Use Only. Not for use in diagnostic procedures.

E Coli

> 90% by SDS-PAGE

Liquid in 20mM Tris pH 8.0, 1mM EDTA, 50mM NaCl

1 mg/ml (determined by Bradford assay) (lot specific)

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of 14-3-3 beta recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The 14-3-3 family plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 beta, a subtype of the 14-3-3 proteins, was found in B Cells, brain and liver etc. This 14-3-3 beta has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Recombinant human 14-3-3 beta was expressed in E Coli and purified by using conventional chromatography techniques.

Signal Transduction

SDS-PAGE

28 kDa (246 aa), confirmed by MALDI-TOF.

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta

14-3-3 protein beta/alpha

14-3-3 protein beta/alpha

KCIP-1??[Similar Products]

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.

Rodriguez LG., et al. (2005) J Cell Physiol. 202 (1):285-94.; Mils V., et al. (2000) Oncogene. 19 (10):1257-65.;

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