Full Product Name
ACTN2 antibody - C-terminal region
Product Gene Name
anti-ACTN2 antibody
[Similar Products]
Product Synonym Gene Name
1110008F24Rik[Similar Products]
Antibody/Peptide Pairs
ACTN2 peptide (MBS3228552) is used for blocking the activity of ACTN2 antibody (MBS3203585)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Synthetic peptide located within the following region: IQSYSIRISS SNPYSTVTMD ELRNKWDKVK QLVPVRDQSL QEELARQHAN
3D Structure
ModBase 3D Structure for Q9JI91
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Purity/Purification
Protein A purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Homology
Cow: 93%; Dog: 93%; Guinea Pig: 93%; Horse: 93%; Human: 93%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Zebrafish: 83%
Immunogen
The immunogen is a synthetic peptide corresponding to a region of Mouse
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-ACTN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACTN2 antibody
This is a rabbit polyclonal antibody against ACTN2. It was validated on Western Blot using a cell lysate as a positive control.
Target Description: The alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibres, while ACTN3 expression is restricted to a subset of type 2 fibres. Murine Actn2 and Actn3 are differentially expressed, spatially and temporally, during embryonic development and, in contrast to humans, alpha-actinin-2 expression does not completely overlap alpha-actinin-3 in postnatal skeletal muscle, suggesting independent function.
Product Categories/Family for anti-ACTN2 antibody
Polyclonal; RNA Binding Proteins; Developmental Biology; DNA/RNA/Protein Interactions;
Applications Tested/Suitable for anti-ACTN2 antibody
Western Blot (WB)
Western Blot (WB) of anti-ACTN2 antibody
WB Suggested Anti-ACTN2 Antibody Titration: 0.625ug/ml
ELISA Titer: 1:312500
Positive Control: Human Muscle
NCBI/Uniprot data below describe general gene information for ACTN2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_150371
[Other Products]
NCBI GenBank Nucleotide #
NM_033268
[Other Products]
UniProt Primary Accession #
Q9JI91
[Other Products]
UniProt Related Accession #
Q9JI91[Other Products]
NCBI Official Full Name
alpha-actinin-2
NCBI Official Synonym Full Names
actinin alpha 2
NCBI Official Symbol
Actn2??[Similar Products]
NCBI Official Synonym Symbols
1110008F24Rik
??[Similar Products]
NCBI Protein Information
alpha-actinin-2
UniProt Protein Name
Alpha-actinin-2
UniProt Synonym Protein Names
Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein
Protein Family
Alpha-actinin
UniProt Gene Name
Actn2??[Similar Products]
UniProt Entry Name
ACTN2_MOUSE
UniProt Comments for ACTN2
ACTN2: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Belongs to the alpha-actinin family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Cellular Component: sarcomere; cortical actin cytoskeleton; focal adhesion; cytoplasm; Z disc; striated muscle thin filament; filopodium
Molecular Function: protein binding, bridging; actin filament binding; protein dimerization activity; identical protein binding; LIM domain binding; protein binding; protein homodimerization activity; ligand-dependent nuclear receptor transcription coactivator activity; metal ion binding; cytoskeletal protein binding; titin binding; calcium ion binding; actin binding; FATZ binding; thyroid hormone receptor coactivator activity
Biological Process: focal adhesion formation; regulation of membrane potential; muscle contraction; negative regulation of potassium ion transport; microspike biogenesis; positive regulation of potassium ion transport; protein homotetramerization
Research Articles on ACTN2
1. Data suggest that kindlin-2 (Kind2/Fermt2) interacts with actin alpha 2 (Actn2) and integrin beta 1 (Itgb1) and co-localizes to cardiac sarcomere at Z-disc; knockdown of Kind2 leads to dissociation of Actn2 and Itgb1.
Precautions
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Disclaimer
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