Anti -Activin Receptor Type IIA (RIIA)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 3; NC_005102.2 (29779827..29808881). Location: 3q12

Polyclonal

IgG

Goat

Recognizes human Activin Receptor Type IIA (RIIA). Shows < 2% crossreactivity with recombinant human Activin RIIB.

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.?

Small volumes of anti-Acvr2a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor-beta (TGF-beta) family members, including Activin, Nodal, Bmp7, Gdf1, Gdf3, Myostatin (Gdf8), and Gdf11. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated.

Antibodies; Abs to Activin Receptors

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Neutralization

Suitable for use in Neutralization, ELISA, Western Blot and Immunohistochemistry.
Dilution: Neutralization: ~1-3ug/ml will block 50% of the binding of 30ng/ml recombinant human Activin A to immobilized recombinant human Act RIIA/Fc Chimera (100ul of a 0.5ug/ml solution was coated in each well) in a functional ELISA assay.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.3ng/well.
Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane and 25ng/lane under non-reducing and reducing conditions, respectively.
Immunohistochemistry (Paraffin): 15ug/ml for 5-15um thick sections. For detection of labeling, the ABC technique using chromogenic substrates is recommended.

57,893 Da[Similar Products]

activin A receptor, type IIA

activin receptor type-2A; ACTR-IIA; activin receptor IIA; type II activin receptor; activin receptor type IIA

Activin receptor type-2A

Actrii; Acvr2??[Similar Products]

AVR2A_RAT

acts as a receptor for activin A and inhibin A [RGD]

ACVR2A: a tyrosine-kinase like receptor kinase of the STKR family. The holoreceptor receptor is a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. Each is composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic kinase domain. Type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptor kinases are apparently constitutively active.

Protein type: Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; Motility/polarity/chemotaxis; Kinase, protein; EC 2.7.11.30; Membrane protein, integral; TKL group; STKR family; Type2 subfamily

Cellular Component: cell surface; cytoplasm; integral to membrane; activin receptor complex; receptor complex

Molecular Function: protein serine/threonine kinase activity; activin receptor activity; protein self-association; growth factor binding; metal ion binding; activin binding; coreceptor activity; activin receptor activity, type II; transmembrane receptor protein serine/threonine kinase activity; ATP binding; receptor signaling protein serine/threonine kinase activity; PDZ domain binding

Biological Process: positive regulation of erythrocyte differentiation; embryonic skeletal development; male gonad development; regulation of BMP signaling pathway; activin receptor signaling pathway; regulation of signal transduction; Sertoli cell proliferation; gastrulation with mouth forming second; positive regulation of bone mineralization; protein amino acid phosphorylation; response to organic substance; anterior/posterior pattern formation; BMP signaling pathway; positive regulation of osteoblast differentiation; positive regulation of activin receptor signaling pathway; mesoderm development; spermatogenesis; positive regulation of protein amino acid phosphorylation; positive regulation of follicle-stimulating hormone secretion; regulation of nitric-oxide synthase activity; sperm ejaculation; determination of left/right symmetry; penile erection

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