Human Membrane primary amine oxidase (AOC3) ELISA kit; HPAO; SSAO; VAP-1; VAP1; amine oxidase; copper containing 3; copper amine oxidase; semicarbazide-sensitive amine oxidase; vascular adhesion protein 1; amine oxidase; copper containing 3 (vascular adhesion protein 1)

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human AOC3. No significant cross-reactivity or interference between Human AOC3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of AOC3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS917881 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the amine oxidase, copper containing 3 (vascular adhesion protein 1) (AOC3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AOC3. The ELISA analytical biochemical technique of the MBS917881 kit is based on AOC3 antibody-AOC3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AOC3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AOC3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AOC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AOC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AOC3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AOC3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

84,622 Da

amine oxidase, copper containing 3

membrane primary amine oxidase; copper amine oxidase; placenta copper monamine oxidase; semicarbazide-sensitive amine oxidase; amine oxidase, copper containing 3 (vascular adhesion protein 1)

Membrane primary amine oxidase

VAP1; SSAO; VAP-1??[Similar Products]

AOC3_HUMAN

This gene encodes a member of the semicarbazide-sensitive amine oxidase family. Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The encoded protein is localized to the cell surface, has adhesive properties as well as monoamine oxidase activity, and may be involved in leukocyte trafficking. Alterations in levels of the encoded protein may be associated with many diseases, including diabetes mellitus. A pseudogene of this gene has been described and is located approximately 9-kb downstream on the same chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]

AOC3: Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin- independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis. Belongs to the copper/topaquinone oxidase family.

Protein type: Cell adhesion; EC 1.4.3.21; Oxidoreductase; Membrane protein, integral; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - glycine, serine and threonine; Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - phenylalanine

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: microvillus; cell surface; cytoplasm; integral to membrane; plasma membrane

Molecular Function: amine oxidase activity; protein binding; protein homodimerization activity; copper ion binding; protein heterodimerization activity; cation channel activity; calcium ion binding; quinone binding

Biological Process: response to antibiotic; amine metabolic process; inflammatory response; cell adhesion; cation transport

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