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fms-related tyrosine kinase 4, ELISA Kit

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產(chǎn)品名稱(chēng): fms-related tyrosine kinase 4, ELISA Kit
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fms-related tyrosine kinase 4, ELISA Kit


fms-related tyrosine kinase 4, ELISA Kit  的詳細(xì)介紹
Product Name

fms-related tyrosine kinase 4 (FLT4), ELISA Kit

Full Product Name

Rat FMS-like tyrosine kinase 3 ligand, Flt-3L ELISA Kit

Product Synonym Names
Rat FMS-like tyrosine kinase 3 ligand; Flt-3L ELISA Kit; FLT41; LMPH1A; PCL; VEGFR3; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; vascular endothelial growth factor receptor 3; fms-related tyrosine kinase 4
Product Gene Name

FLT4 elisa kit

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Request for Current Manual Insert
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3D Structure
ModBase 3D Structure for Q91ZT1
Species Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat FLT4. No significant cross-reactivity or interference between Rat FLT4 and analogues was observed.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FLT4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for FLT4 purchase
MBS702994 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the fms-related tyrosine kinase 4 (FLT4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FLT4. The ELISA analytical biochemical technique of the MBS702994 kit is based on FLT4 antibody-FLT4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FLT4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FLT4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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NCBI/Uniprot data below describe general gene information for FLT4. It may not necessarily be applicable to this product.
NCBI GI #
16758466
NCBI GeneID
114110
NCBI Accession #
NP_446104.1 [Other Products]
NCBI GenBank Nucleotide #
NM_053652.1 [Other Products]
UniProt Primary Accession #
Q91ZT1 [Other Products]
UniProt Secondary Accession #
O35755; Q91ZT0[Other Products]
UniProt Related Accession #
Q91ZT1[Other Products]
Molecular Weight
153,317 Da
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NCBI Official Full Name
vascular endothelial growth factor receptor 3
NCBI Official Synonym Full Names
fms-related tyrosine kinase 4
NCBI Official Symbol
Flt4??[Similar Products]
NCBI Official Synonym Symbols
Vegfr3
??[Similar Products]
NCBI Protein Information
vascular endothelial growth factor receptor 3; FLT-4; VEGFR-3; FMS-like tyrosine kinase 4; tyrosine-protein kinase receptor FLT4
UniProt Protein Name
Vascular endothelial growth factor receptor 3
UniProt Synonym Protein Names
Fms-like tyrosine kinase 4; FLT-4; Tyrosine-protein kinase receptor FLT4
Protein Family
Vascular endothelial growth factor receptor
UniProt Gene Name
Flt4??[Similar Products]
UniProt Synonym Gene Names
Flt-4; Vegfr3; VEGFR-3; FLT-4??[Similar Products]
UniProt Entry Name
VGFR3_RAT
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NCBI Summary for FLT4
VEGF receptor-related, cell surface associated kinase with growth promoting ability [RGD, Feb 2006]
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UniProt Comments for FLT4
Function: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in ***** lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulation: Present in an inactive conformation in the absence of bound ligand. Binding of VEGFC or VEGFD leads to dimerization and activation by autophosphorylation on tyrosine residues
Research Articles on FLT4
1. Expression of VEGFR-3 is enhanced in various cell types of the infarcted heart.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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