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GATA Binding Protein 2, ELISA Kit

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GATA Binding Protein 2, ELISA Kit


GATA Binding Protein 2, ELISA Kit  的詳細(xì)介紹
Product Name

GATA Binding Protein 2 (GATA2), ELISA Kit

Full Product Name

Rabbit GATA Binding Protein 2 ELISA Kit

Product Gene Name

GATA2 elisa kit

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
Request for Current Manual Insert
Request Current Manual
OMIM
614286
Species Reactivity
Rabbit
Specificity
This assay has high sensitivity and excellent specificity for detection of GATA2. No significant cross-reactivity or interference between GATA2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GATA2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This GATA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit GATA2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GATA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for GATA2 purchase
MBS733288 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GATA Binding Protein 2 (GATA2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GATA2. The ELISA analytical biochemical technique of the MBS733288 kit is based on GATA2 antibody-GATA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GATA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GATA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for
GATA2 elisa kit
Principle of the assay: GATA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GATA2 antibody and an GATA2-HRP conjugate. The assay sample and buffer are incubated together with GATA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GATA2 concentration since GATA2 from samples and GATA2-HRP conjugate compete for the anti-GATA2 antibody . Since the number of sites is limited, as more sites are occupied by GATA2 from the sample, fewer sites are left to bind GATA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GATA2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for GATA2 elisa kit
Signal Transduction

Typical Testing Data/Standard Curve (for reference only) of GATA2 elisa kit
GATA2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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NCBI/Uniprot data below describe general gene information for GATA2. It may not necessarily be applicable to this product.
NCBI GI #
30410878
NCBI GeneID
2624
NCBI Accession #
AAH51272.1 [Other Products]
UniProt Secondary Accession #
Q53YE0; Q96BH0; Q96BH8; Q9BUJ6; D3DNB3[Other Products]
UniProt Related Accession #
P23769[Other Products]
Molecular Weight
49,106 Da
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NCBI Official Full Name
GATA2 protein
NCBI Official Synonym Full Names
GATA binding protein 2
NCBI Official Symbol
GATA2??[Similar Products]
NCBI Official Synonym Symbols
DCML; IMD21; NFE1B; MONOMAC
??[Similar Products]
NCBI Protein Information
endothelial transcription factor GATA-2
UniProt Protein Name
Endothelial transcription factor GATA-2
UniProt Synonym Protein Names
GATA-binding protein 2
Protein Family
GATA-binding factor
UniProt Gene Name
GATA2??[Similar Products]
UniProt Entry Name
GATA2_HUMAN
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NCBI Summary for GATA2
This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]
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UniProt Comments for GATA2
GATA2: Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'. Endothelial cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: nucleoplasm; protein complex

Molecular Function: protein binding; zinc ion binding; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: embryonic placenta development; somatic stem cell maintenance; cell maturation; cell fate determination; negative regulation of transcription from RNA polymerase II promoter; cell differentiation in hindbrain; positive regulation of phagocytosis; positive regulation of megakaryocyte differentiation; central nervous system neuron development; inner ear morphogenesis; transcription, DNA-dependent; positive regulation of erythrocyte differentiation; regulation of histone acetylation; negative regulation of fat cell differentiation; phagocytosis; positive regulation of angiogenesis; negative regulation of macrophage differentiation; pituitary gland development; ventral spinal cord interneuron differentiation; homeostasis of number of cells within a tissue; commitment of a neuronal cell to a specific type of neuron in the forebrain; negative regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; urogenital system development

Disease: Lymphedema, Primary, With Myelodysplasia; Immunodeficiency 21; Myelodysplastic Syndrome
Research Articles on GATA2
1. RNAi-mediated knockdown of GATA2 expression significantly enhanced proliferation.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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