Anti -Activin Receptor Type IIA (RIIA)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 3; NC_005102.2 (29779827..29808881). Location: 3q12

Monoclonal

IgG2a

9H20

Mouse

Recognizes human Activin RIIA. Does not crossreact with recombinant human Activin RIA, recombinant human Activin RIB or recombinant human Activin RIIB.

Purified by Protein A affinity chromatography from hybridoma culture supernatant.

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.

~0.5mg/ml (after reconsitution) (lot specific)

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-Acvr2a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A) and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin, but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP-2/7-bound BMPR-II. Activin type II receptors are highly conserved. Human, mouse and rat type II activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type II receptors bind activin with high affinity, and are potent activin antagonists.

Antibodies; Abs to Activin Receptors

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1ug/ml using human testis tissue.

Western Blot detection of human Activin RIIA in lysates of human testis tissue using MBS606695. PVDF Membrane was probed with 1ug/ml of MBS606695 followed by IgG (HRP) anti-mouse secondary antibody. A specific band was detected for Activin RIIA at ~65kD (as indicated). This experiment was conducted under reducing conditions.

57,893 Da[Similar Products]

activin A receptor, type IIA

activin receptor type-2A; ACTR-IIA; activin receptor IIA; type II activin receptor; activin receptor type IIA

Activin receptor type-2A

Actrii; Acvr2??[Similar Products]

AVR2A_RAT

acts as a receptor for activin A and inhibin A [RGD]

ACVR2A: a tyrosine-kinase like receptor kinase of the STKR family. The holoreceptor receptor is a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. Each is composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic kinase domain. Type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptor kinases are apparently constitutively active.

Protein type: Membrane protein, integral; EC 2.7.11.30; Protein kinase, TKL; Motility/polarity/chemotaxis; Protein kinase, Ser/Thr (receptor); Kinase, protein; TKL group; STKR family; Type2 subfamily

Cellular Component: cell surface; cytoplasm; integral to membrane; activin receptor complex; receptor complex

Molecular Function: protein serine/threonine kinase activity; activin receptor activity; protein self-association; metal ion binding; growth factor binding; activin binding; coreceptor activity; activin receptor activity, type II; ATP binding; transmembrane receptor protein serine/threonine kinase activity; PDZ domain binding; receptor signaling protein serine/threonine kinase activity

Biological Process: embryonic skeletal development; positive regulation of erythrocyte differentiation; male gonad development; regulation of BMP signaling pathway; activin receptor signaling pathway; regulation of signal transduction; Sertoli cell proliferation; gastrulation with mouth forming second; positive regulation of bone mineralization; protein amino acid phosphorylation; response to organic substance; BMP signaling pathway; anterior/posterior pattern formation; positive regulation of osteoblast differentiation; positive regulation of activin receptor signaling pathway; mesoderm development; spermatogenesis; positive regulation of protein amino acid phosphorylation; positive regulation of follicle-stimulating hormone secretion; regulation of nitric-oxide synthase activity; sperm ejaculation; determination of left/right symmetry; penile erection

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