Full Product Name
BCOR siRNA (Mouse)
Product Synonym Names
KIAA1575; BCL-6 corepressor; BCoR
Product Gene Name
BCOR sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8CGN4
Specificity
BCOR siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BCOR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of BCOR sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
BCOR sirna
siRNA to inhibit BCOR expression using RNA interference
Applications Tested/Suitable for BCOR sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for BCOR. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001161793.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001168321.1
[Other Products]
UniProt Primary Accession #
Q8CGN4
[Other Products]
UniProt Secondary Accession #
Q6PDK5; Q6ZPM3; Q8BKF5; Q8CGN1; Q8CGN2; Q8CGN3; B1AXK3; B1AXK4; B1AXK5; B1AXK6[Other Products]
UniProt Related Accession #
Q8CGN4[Other Products]
Molecular Weight
186,710 Da
NCBI Official Full Name
BCL-6 corepressor isoform d
NCBI Official Synonym Full Names
BCL6 interacting corepressor
NCBI Official Symbol
Bcor??[Similar Products]
NCBI Official Synonym Symbols
BcorR; mKIAA1575; 5830466J11Rik; 8430401K06Rik; D930024N20Rik
??[Similar Products]
NCBI Protein Information
BCL-6 corepressor
UniProt Protein Name
BCL-6 corepressor
Protein Family
BCL-6 corepressor
UniProt Gene Name
Bcor??[Similar Products]
UniProt Synonym Gene Names
Kiaa1575; BCoR??[Similar Products]
UniProt Entry Name
BCOR_MOUSE
UniProt Comments for BCOR
BCOR: Transcriptional corepressor. May specifically inhibit gene expression when recruited to promoter regions by sequence specific DNA-binding proteins such as BCL6 and MLLT3. This repression may be mediated at least in part by histone deacetylase activities which can associate with this corepressor. Involved in the repression of TFAP2A; impairs binding of BCL6 and KDM2B to TFAP2A promoter regions. Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in ***** stem cells; the function implies inhibition of methylation on histone H3 'Lys-4' (H3K4me3) and 'Lys-36' (H3K36me2). Defects in BCOR are the cause of microphthalmia syndromic type 2 (MCOPS2). Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS2 is a very rare multiple congenital anomaly syndrome characterized by eye anomalies (congenital cataract, microphthalmia, or secondary glaucoma), facial abnormalities (long narrow face, high nasal bridge, pointed nose with cartilages separated at the tip, cleft palate, or submucous cleft palate), cardiac anomalies (atrial septal defect, ventricular septal defect, or floppy mitral valve) and dental abnormalities (canine radiculomegaly, delayed dentition, oligodontia, persistent primary teeth, or variable root length). Belongs to the BCOR family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor
Cellular Component: PcG protein complex; nucleus
Molecular Function: protein binding; heat shock protein binding; histone deacetylase binding; ubiquitin-protein ligase activity; transcription factor binding; transcription corepressor activity
Biological Process: odontogenesis; negative regulation of bone mineralization; transcription, DNA-dependent; negative regulation of histone H3-K36 methylation; regulation of transcription, DNA-dependent; heart development; specification of axis polarity; negative regulation of histone H3-K4 methylation; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; palate development; negative regulation of transcription, DNA-dependent
Research Articles on BCOR
1. T cell-expressed BCOR is critical for optimal GC-Tfh cell differentiation and humoral immunity.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
