Product Name
ALMS1, siRNA
Full Product Name
ALMS1 siRNA (Human)
Product Synonym Names
KIAA0328; Alstrom syndrome protein 1
Product Gene Name
ALMS1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8TCU4
Specificity
ALMS1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ALMS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ALMS1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ALMS1 sirna
siRNA to inhibit ALMS1 expression using RNA interference
Applications Tested/Suitable for ALMS1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ALMS1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_055935.4
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NCBI GenBank Nucleotide #
NM_015120.4
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UniProt Primary Accession #
Q8TCU4
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UniProt Secondary Accession #
Q53S05; Q580Q8; Q86VP9; Q9Y4G4[Other Products]
UniProt Related Accession #
Q8TCU4[Other Products]
Molecular Weight
425,137 Da
NCBI Official Full Name
Alstrom syndrome protein 1
NCBI Official Synonym Full Names
Alstrom syndrome protein 1
NCBI Official Symbol
ALMS1??[Similar Products]
NCBI Official Synonym Symbols
ALSS
??[Similar Products]
NCBI Protein Information
Alstrom syndrome protein 1
UniProt Protein Name
Alstrom syndrome protein 1
Protein Family
Alstrom syndrome protein
UniProt Gene Name
ALMS1??[Similar Products]
UniProt Synonym Gene Names
KIAA0328??[Similar Products]
UniProt Entry Name
ALMS1_HUMAN
NCBI Summary for ALMS1
This gene encodes a protein containing a large tandem-repeat domain as well as additional low complexity regions. The encoded protein functions in microtubule organization, particularly in the formation and maintanance of cilia. Mutations in this gene cause Alstrom syndrome. There is a pseudogene for this gene located adjacent in the same region of chromosome 2. Alternative splice variants have been described but their full length nature has not been determined. [provided by RefSeq, Apr 2014]
UniProt Comments for ALMS1
ALMS1: Involved in PCM1-dependent intracellular transport. Required, directly or indirectly, for the localization of NCAPD2 to the proximal ends of centrioles. Required for proper formation and/or maintenance of primary cilia (PC), microtubule-based structures that protrude from the surface of epithelial cells. Defects in ALMS1 are the cause of Alstrom syndrome (ALMS). Alstrom syndrome is a rare autosomal recessive disorder characterized by progressive cone-rod retinal dystrophy, neurosensory hearing loss, early childhood obesity and type 2 diabetes mellitus. Dilated cardiomyopathy, acanthosis nigricans, male hypogonadism, hypothyroidism, developmental delay and hepatic dysfunction can also be associated with the syndrome. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation
Chromosomal Location of Human Ortholog: 2p13
Cellular Component: centriole; nucleoplasm; spindle pole; centrosome; cytoplasm; cytosol; nucleus; cilium
Molecular Function: protein binding
Biological Process: organelle organization and biogenesis; regulation of stress fiber formation; mitotic cell cycle; G2/M transition of mitotic cell cycle; endosome transport
Disease: Alstrom Syndrome
Research Articles on ALMS1
1. Identification of a homozygous deleterious mutation in the ALMS1 gene as the cause of mitogenic cardiomyopathy in two siblings.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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