Anti -ALMS1 (Alstrom syndrome 1, ALSS, KIAA0328)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 2; NC_000002.11 (73612886..73837046). Location: 2p13

Polyclonal

Goat

Recognizes human ALMS1 at ~200kD and 75kD in lysates of human T cell line Jurkat after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 461kD according to NP_055935.3. Species Crossreactivity: Canine, mouse and rat.

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-ALMS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Antibodies; Abs to Disease Markers

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)

Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: 1:32000
Western Blot: 0.3ug/ml. Preliminary experiments gave bands at approx 200kD and 75kD in lysates of Human T cell line Jurkat after antibody staining. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

460,965 Da[Similar Products]

Alstrom syndrome 1

Alstrom syndrome protein 1; OTTHUMP00000202344; OTTHUMP00000202401

Alstrom syndrome protein 1

KIAA0328??[Similar Products]

ALMS1_HUMAN

This gene encodes a protein containing a large tandem-repeat domain. The mouse ortholog of this gene has been shown to function in ciliogenesis in inner medullary collecting duct cells. Mutations in this gene have been associated with Alstrom syndrome. Alternative splice variants have been described but their full length sequences have not been determined.

ALMS1: Involved in PCM1-dependent intracellular transport. Required, directly or indirectly, for the localization of NCAPD2 to the proximal ends of centrioles. Required for proper formation and/or maintenance of primary cilia (PC), microtubule-based structures that protrude from the surface of epithelial cells. Defects in ALMS1 are the cause of Alstrom syndrome (ALMS). Alstrom syndrome is a rare autosomal recessive disorder characterized by progressive cone-rod retinal dystrophy, neurosensory hearing loss, early childhood obesity and type 2 diabetes mellitus. Dilated cardiomyopathy, acanthosis nigricans, male hypogonadism, hypothyroidism, developmental delay and hepatic dysfunction can also be associated with the syndrome. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: nucleoplasm; centriole; spindle pole; centrosome; cytoplasm; cytosol; nucleus; cilium

Molecular Function: protein binding

Biological Process: regulation of stress fiber formation; organelle organization and biogenesis; mitotic cell cycle; endosome transport; G2/M transition of mitotic cell cycle

Disease: Alstrom Syndrome

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.