USH1F; DFNB23; CDHR15; Deafness, Autosomal Recessive 23; Cadherin-Related Family Member 15

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against PCDH15. It has been selected for its ability to recognize PCDH15 in immunohistochemical staining and western blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Small volumes of anti-PCDH15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot: Sample: Recombinant PCDH15, Mouse.

185,901 Da

protocadherin 15

protocadherin-15

Protocadherin-15

PCDH15: Calcium-dependent cell-adhesion protein. Essential for maintenance of normal retinal and cochlear function. Defects in PCDH15 are the cause of Usher syndrome type 1F (USH1F). USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa and sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH1 is characterized by profound congenital sensorineural deafness, absent vestibular function and prepubertal onset of progressive retinitis pigmentosa leading to blindness. Defects in PCDH15 are a cause of Usher syndrome type 1D/F (USH1DF). USH1DF patients are heterozygous for mutations in CDH23 and PCDH15, indicating a digenic inheritance pattern. Defects in PCDH15 are the cause of deafness autosomal recessive type 23 (DFNB23). DFNB23 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Cell development/differentiation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10 B5.3|10 37.43 cM

Cellular Component: cytoplasm; extracellular region; extracellular space; integral component of membrane; integral component of plasma membrane; membrane; photoreceptor outer segment; plasma membrane; stereocilium; stereocilium bundle; synapse

Molecular Function: calcium ion binding; protein binding; protein complex binding; protein N-terminus binding

Biological Process: actin filament bundle formation; actin filament organization; ***** locomotory behavior; ***** walking behavior; auditory receptor cell differentiation; auditory receptor cell stereocilium organization and biogenesis; cell adhesion; cell-cell signaling; detection of mechanical stimulus involved in equilibrioception; detection of mechanical stimulus involved in sensory perception of sound; equilibrioception; homophilic cell adhesion; inner ear development; inner ear receptor stereocilium organization and biogenesis; locomotory behavior; morphogenesis of an epithelium; multicellular organism growth; photoreceptor cell maintenance; response to calcium ion; righting reflex; sensory perception of light stimulus; sensory perception of sound; startle response; visual perception

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