Product Name
Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a), ELISA Kit
Full Product Name
Rat Amiloride Sensitive Sodium Channel Subunit Alpha ELISA Kit (SCNN1a)
Product Gene Name
SCNN1a elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
3D Structure
ModBase 3D Structure for P37089
Assay Type
Sandwich ELISA
Samples
Cell Culture Supernatant, Serum, Plasma (EDTA, Citrate, Heparin)
Detection Range
0.156-10 ng/mL
Preparation and Storage
Store at 4 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SCNN1a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SCNN1a purchase
MBS9139463 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) ELISA Kit target analytes in
biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SCNN1a. The ELISA analytical biochemical technique of the MBS9139463 kit is based on SCNN1a antibody-SCNN1a antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SCNN1a antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SCNN1a. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SCNN1a elisa kit
Product Overview: The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, heparin).
Intended Use: This Rat Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Rat Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) in the sample, this Rat Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Rat Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI/Uniprot data below describe general gene information for SCNN1a. It may not necessarily be applicable to this product.
NCBI Accession #
NP_113736.1
[Other Products]
NCBI GenBank Nucleotide #
NM_031548.2
[Other Products]
UniProt Primary Accession #
P37089
[Other Products]
UniProt Secondary Accession #
Q64593; Q9QUI4[Other Products]
UniProt Related Accession #
P37089[Other Products]
Molecular Weight
78,888 Da
NCBI Official Full Name
amiloride-sensitive sodium channel subunit alpha
NCBI Official Synonym Full Names
sodium channel epithelial 1 alpha subunit
NCBI Official Symbol
Scnn1a??[Similar Products]
NCBI Official Synonym Symbols
ENaCA
??[Similar Products]
NCBI Protein Information
amiloride-sensitive sodium channel subunit alpha
UniProt Protein Name
Amiloride-sensitive sodium channel subunit alpha
UniProt Synonym Protein Names
Alpha-NaCH; Epithelial Na(+) channel subunit alpha; Alpha-ENaC; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA
Protein Family
Amiloride-sensitive sodium channel
UniProt Gene Name
Scnn1a??[Similar Products]
UniProt Synonym Gene Names
Renac; Alpha-ENaC??[Similar Products]
NCBI Summary for SCNN1a
acts as an epithelial sodium ion channel; regulates salt and fluid transport in the kidney [RGD, Feb 2006]
UniProt Comments for SCNN1a
Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells (PubMed:8382172). Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and eccrine sweat glands. Also plays a role in taste perception ().
Research Articles on SCNN1a
1. Studied the effect and underlining mechanisms of MaR1(maresin 1) in modulating alveolar fluid clearance on LPS-induced acute lung injury; found MaR1 improved epithelial sodium channel (ENaC) and Na,K-adenosine triphosphatase expression and activity.
Precautions
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Disclaimer
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