Mouse Monoclonal [clone 2E2] (IgG2a) to Human COMMD1; Human COMMD1; C2orf5; Protein Murr1; Copper metabolism gene MURR1; MURR1

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a

2E2

Mouse

Human COMMD1

Protein A/G purified

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

1 mg/ml (lot specific)

Store at -20 degree C. Minimize freezing and thawing.

Small volumes of anti-COMMD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

COMMD1 Antibody, C2orf5 Antibody, Protein Murr1 Antibody, Copper metabolism gene MURR1 Antibody, MURR1 Antibody Description: Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity. Down-regulates SOD1 activity by interfering with its homodimerization. Plays a role in copper ion homeostasis. Can bind one copper ion per monomer. May function to facilitate biliary copper excretion within hepatocytes.

Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)

Flow Cytometry (1:100)
IHC-P (10 ug/ml)
WB (1:2000)

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COMMD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COMMD1.

Western blot of extracts (35 ug) from 9 different cell lines by using g anti-COMMD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Flow cytometry of HeLa cells, using anti-COMMD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-COMMD1 antibody, and then analyzed by flow cytometry.

Flow cytometry of Jurkat cells, using anti-COMMD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

17,665 Da

copper metabolism domain containing 1

COMM domain-containing protein 1

COMM domain-containing protein 1

C2orf5; MURR1??[Similar Products]

COMD1_HUMAN

COMMD1 is a regulator of copper homeostasis, sodium uptake, and NF-kappa-B (see MIM 164011) signaling (de Bie et al., 2005 [PubMed 16267171]).[supplied by OMIM, Sep 2009]

COMMD1: Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity. Down-regulates SOD1 activity by interfering with its homodimerization. Plays a role in copper ion homeostasis. Can bind one copper ion per monomer. May function to facilitate biliary copper excretion within hepatocytes.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 2p15

Cellular Component: Cul2-RING ubiquitin ligase complex; cytoplasm; cytoplasmic membrane-bound vesicle; early endosome; endosome membrane; nucleoplasm; nucleus; recycling endosome

Molecular Function: copper ion binding; identical protein binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; protein homodimerization activity

Biological Process: copper ion homeostasis; Golgi to plasma membrane transport; inhibition of NF-kappaB transcription factor; plasma membrane to endosome transport; positive regulation of protein ubiquitination; protein transport; regulation of proteasomal ubiquitin-dependent protein catabolic process; transcription, DNA-dependent

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