Full Product Name
LRAT Antibody
Product Synonym Names
Lecithin retinol acyltransferase; EC 2.3.1.135; Phosphatidylcholine--retinol O-acyltransferase; LRAT
Product Gene Name
anti-LRAT antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O95237
Species Reactivity
Human, Mouse, Rat
Specificity
LRAT Antibody detects endogenous levels of total LRAT
Purity/Purification
Immunogen affinity purified
Form/Format
In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
A synthesized peptide
Preparation and Storage
Store at-20 degree C for 1 year
Other Notes
Small volumes of anti-LRAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-LRAT antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-LRAT antibody
WB: 1:500-1:1000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
Western Blot (WB) of anti-LRAT antibody
Western blot analysis of extracts from COLO205 cells, using LRAT antibody.
NCBI/Uniprot data below describe general gene information for LRAT. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001288574.1
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NCBI GenBank Nucleotide #
NM_001301645.1
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UniProt Primary Accession #
O95237
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UniProt Secondary Accession #
Q8N716; A8K983[Other Products]
UniProt Related Accession #
O95237[Other Products]
NCBI Official Full Name
lecithin retinol acyltransferase
NCBI Official Synonym Full Names
lecithin retinol acyltransferase
NCBI Official Symbol
LRAT??[Similar Products]
NCBI Official Synonym Symbols
LCA14
??[Similar Products]
NCBI Protein Information
lecithin retinol acyltransferase
UniProt Protein Name
Lecithin retinol acyltransferase
UniProt Synonym Protein Names
Phosphatidylcholine--retinol O-acyltransferase
Protein Family
Lecithin retinol acyltransferase
UniProt Gene Name
LRAT??[Similar Products]
NCBI Summary for LRAT
The protein encoded by this gene localizes to the endoplasmic reticulum, where it catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester. This reaction is an important step in vitamin A metabolism in the visual system. Mutations in this gene have been associated with early-onset severe retinal dystrophy and Leber congenital amaurosis 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]
UniProt Comments for LRAT
Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments.
Research Articles on LRAT
1. instability of LRAT(E14L) did not abrogate the production of the visual chromophore in a cell-based assay. Instead, expression of LRAT(E14L) led to a rapid increase in cellular levels of retinoic acid upon retinoid supplementation.
Precautions
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Disclaimer
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