Full Product Name
DNM1 Antibody
Product Synonym Names
Dynamin-1, DNM
Product Gene Name
anti-DNM1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human, Mouse, Rat
Specificity
The antibody detects endogenous level of total DNM1 protein.
Form/Format
Supplied at 1.4mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
Immunogen Type
Peptide-KLH
Immunogen Description
Synthetic peptide corresponding to a region derived from 850-863 amino acids of Human Dynamin-1
Preparation and Storage
Store at -20 degree C/1 year
Other Notes
Small volumes of anti-DNM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DNM1 antibody
This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described.
Product Categories/Family for anti-DNM1 antibody
Total protein Ab
Applications Tested/Suitable for anti-DNM1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-DNM1 antibody
ELISA: 1:1000-1:5000
Western blotting: 1:500-1:2000
Testing Data of anti-DNM1 antibody
Gel: 8%SDS-PAGE Lysate: 40 ug Mouse brain tissue lysate Primary antibody: 1/700 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/5000 dilution Exposure time: 30 seconds
NCBI/Uniprot data below describe general gene information for DNM1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_004399
[Other Products]
NCBI GenBank Nucleotide #
NM_004408.3
[Other Products]
UniProt Secondary Accession #
Q5SYX0; Q5SYX2; Q6P3T6; Q86VD2; A6NLM6[Other Products]
UniProt Related Accession #
Q05193[Other Products]
Molecular Weight
95,895 Da
NCBI Official Full Name
dynamin-1 isoform 1
NCBI Official Synonym Full Names
dynamin 1
NCBI Official Symbol
DNM1??[Similar Products]
NCBI Official Synonym Symbols
DNM; EIEE31
??[Similar Products]
NCBI Protein Information
dynamin-1
UniProt Protein Name
Dynamin-1
UniProt Gene Name
DNM1??[Similar Products]
UniProt Synonym Gene Names
DNM??[Similar Products]
UniProt Entry Name
DYN1_HUMAN
NCBI Summary for DNM1
This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Comments for DNM1
DYN1: a microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Concentrates within the presynaptic compartment and may participate in specialized neuronal functions such as rapid synaptic vesicle recycling. Part of a protein network that controls nucleation of actin from membranes. Contains one PH domain.
Protein type: Motility/polarity/chemotaxis; Vesicle; Motor; Hydrolase; Microtubule-binding; EC 3.6.5.5
Chromosomal Location of Human Ortholog: 9q34
Cellular Component: microtubule; membrane coat; plasma membrane
Molecular Function: protein C-terminus binding; GTPase activity; identical protein binding; protein binding; GTP binding; protein complex binding; nitric-oxide synthase binding; protein kinase binding
Biological Process: axon guidance; receptor-mediated endocytosis; endosome organization and biogenesis; receptor internalization; ephrin receptor signaling pathway; endocytosis
Disease: Epileptic Encephalopathy, Early Infantile, 31
Research Articles on DNM1
1. Data suggest that by binding to both clathrin and F-actin, mammalian actin-binding protein 1 (mAbp1; HIP-55 or SH3P7) is specifically recruited at a late stage of clathrin-coated pits (CCPs) formation, which subsequently recruits dynamin to CCPs.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
