Product Name
CYTOKERATIN 14 (KRT14), Monoclonal Antibody
Full Product Name
MOUSE ANTI HUMAN CYTOKERATIN 14
Product Gene Name
anti-KRT14 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 17; NC_000017.10 (39738531..39743147, complement). Location: 17q12-q21
3D Structure
ModBase 3D Structure for P02533
Specificity
CYTOKERATIN 14
This item recognises cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express cytokeratin 14.
Clone LL002 has been reported to be suitable for use in Western blotting.
Form/Format
Ready To Use (Purified IgG - liquid)
Immunogen
Last 15 C-terminal residues of human cytokeratin 14 conjugated to thyroglobulin.
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Preservative Stabilizers
0.09% Sodium Azide
0.5% Bovine Serum Albumin
Buffer Solution
Phosphate buffered saline
Preparation and Storage
Store at +4 degree C.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of dispatch.
Other Notes
Small volumes of anti-KRT14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-KRT14 antibody
Immunohistology - Frozen (IHC), Immunofluorescence (IF), Immunohistology - Paraffin (IHC)
Application Notes for anti-KRT14 antibody
Immunohistology - Paraffin: Max Dilution: Neat
Application Note: Membrane permeabilisation is required for this application. We recommend the use of Leucoperm for this purpose.
Histology Positive Control Tissue: Skin
Immunohistology: * This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Enzyme digestion is not recommended.
Testing Data #1 of anti-KRT14 antibody
Immunoperoxidase staining of formalin fixed, paraffin embedded human tonsil with Mouse anti Human cytokeratin 14 (MBS213190) folllowing heat mediated antigen retrieval using citrate buffer, pH6.0
NCBI/Uniprot data below describe general gene information for KRT14. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000517.2
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NCBI GenBank Nucleotide #
NM_000526.4
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UniProt Primary Accession #
P02533
[Other Products]
UniProt Secondary Accession #
Q14715; Q53XY3; Q9BUE3; Q9UBN2; Q9UBN3; Q9UCY4[Other Products]
UniProt Related Accession #
P02533[Other Products]
Molecular Weight
51,561 Da[Similar Products]
NCBI Official Full Name
keratin, type I cytoskeletal 14
NCBI Official Synonym Full Names
keratin 14
NCBI Official Symbol
KRT14??[Similar Products]
NCBI Official Synonym Symbols
K14; NFJ; CK14; EBS3; EBS4
??[Similar Products]
NCBI Protein Information
keratin, type I cytoskeletal 14; CK-14; keratin-14; cytokeratin 14; cytokeratin-14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
UniProt Protein Name
Keratin, type I cytoskeletal 14
UniProt Synonym Protein Names
Cytokeratin-14; CK-14; Keratin-14
UniProt Gene Name
KRT14??[Similar Products]
UniProt Synonym Gene Names
CK-14; K14??[Similar Products]
UniProt Entry Name
K1C14_HUMAN
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
