Full Product Name
LRAT Polyclonal Antibody
Product Synonym Names
LRAT; Lecithin retinol acyltransferase; Phosphatidylcholine--retinol O-acyltransferase
Product Gene Name
anti-LRAT antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Antigen Affinity Purified
Concentration
1 mg/ml (lot specific)
Immunogen
Synthesized peptide derived from the Internal region of human LRAT.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-49223 / sc-99016
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-LRAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-LRAT antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-LRAT antibody
WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:50-1:100, ELISA: 1:5000-1:20000
Western Blot (WB) of anti-LRAT antibody
Western Blot analysis of various cells using LRAT Polyclonal Antibody at dilution of 1:500.
NCBI/Uniprot data below describe general gene information for LRAT. It may not necessarily be applicable to this product.
NCBI Accession #
NP_004735.2
[Other Products]
NCBI GenBank Nucleotide #
NM_004744.4
[Other Products]
UniProt Secondary Accession #
Q8N716; A8K983[Other Products]
UniProt Related Accession #
O95237[Other Products]
Molecular Weight
Calculated MW: 26
Observed MW: 27
NCBI Official Full Name
lecithin retinol acyltransferase
NCBI Official Synonym Full Names
lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)
NCBI Official Symbol
LRAT??[Similar Products]
NCBI Official Synonym Symbols
LCA14
??[Similar Products]
NCBI Protein Information
lecithin retinol acyltransferase
UniProt Protein Name
Lecithin retinol acyltransferase
UniProt Synonym Protein Names
Phosphatidylcholine--retinol O-acyltransferase
Protein Family
Lecithin retinol acyltransferase
UniProt Gene Name
LRAT??[Similar Products]
UniProt Entry Name
LRAT_HUMAN
NCBI Summary for LRAT
The protein encoded by this gene localizes to the endoplasmic reticulum, where it catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester. This reaction is an important step in vitamin A metabolism in the visual system. Mutations in this gene have been associated with early-onset severe retinal dystrophy and Leber congenital amaurosis 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]
UniProt Comments for LRAT
LRAT: Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments. Defects in LRAT are a cause of Leber congenital amaurosis type 14 (LCA14). It is a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus. Belongs to the H-rev107 family.
Protein type: Cofactor and Vitamin Metabolism - retinol; EC 2.3.1.135; Transferase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 4q32.1
Cellular Component: endoplasmic reticulum membrane; integral to membrane; multivesicular body; perinuclear region of cytoplasm; rough endoplasmic reticulum
Molecular Function: phosphatidylcholine-retinol O-acyltransferase activity; retinoic acid binding; retinol binding; transferase activity, transferring acyl groups
Biological Process: fat-soluble vitamin metabolic process; phototransduction, visible light; positive regulation of lipid transport; retinoic acid metabolic process; retinoid metabolic process; retinol metabolic process; visual perception; vitamin A metabolic process; vitamin metabolic process
Disease: Leber Congenital Amaurosis 14; Retinitis Pigmentosa
Research Articles on LRAT
1. LRAT hypermethylation was associated with decreased mRNA levels in colorectal cancer clinical specimens.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
