Full Product Name
LRAT siRNA (Mouse)
Product Synonym Names
Lecithin retinol acyltransferase; Phosphatidylcholine--retinol O-acyltransferase; Phosphatidylcholine-retinol-O-acyltransferase
Product Gene Name
LRAT sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9JI60
Specificity
LRAT siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse LRAT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of LRAT sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
LRAT sirna
siRNA to inhibit LRAT expression using RNA interference
Applications Tested/Suitable for LRAT sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for LRAT. It may not necessarily be applicable to this product.
NCBI Accession #
NP_076113.1
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NCBI GenBank Nucleotide #
NM_023624.4
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UniProt Primary Accession #
Q9JI60
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UniProt Related Accession #
Q9JI60[Other Products]
Molecular Weight
25,820 Da
NCBI Official Full Name
lecithin retinol acyltransferase
NCBI Official Synonym Full Names
lecithin-retinol acyltransferase (phosphatidylcholine-retinol-O-acyltransferase)
NCBI Official Symbol
Lrat??[Similar Products]
NCBI Official Synonym Symbols
AI449251; 1300010A18Rik
??[Similar Products]
NCBI Protein Information
lecithin retinol acyltransferase
UniProt Protein Name
Lecithin retinol acyltransferase
UniProt Synonym Protein Names
Phosphatidylcholine--retinol O-acyltransferase; Phosphatidylcholine-retinol-O-acyltransferase
Protein Family
Lecithin retinol acyltransferase
UniProt Gene Name
Lrat??[Similar Products]
UniProt Entry Name
LRAT_MOUSE
UniProt Comments for LRAT
LRAT: Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments. Defects in LRAT are a cause of Leber congenital amaurosis type 14 (LCA14). It is a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus. Belongs to the H-rev107 family.
Protein type: Transferase; Membrane protein, integral; EC 2.3.1.135; Cofactor and Vitamin Metabolism - retinol
Cellular Component: multivesicular body; rough endoplasmic reticulum; membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; integral to membrane; endosome
Molecular Function: transferase activity; retinoic acid binding; transferase activity, transferring acyl groups; O-acyltransferase activity; retinol binding; phosphatidylcholine-retinol O-acyltransferase activity
Biological Process: positive regulation of lipid transport; embryonic development; visual perception; retinol metabolic process; response to stimulus; lecithin metabolic process; vitamin A metabolic process
Research Articles on LRAT
1. Mislocalized M-opsin was degraded whereas mislocalized S-opsin accumulated in Lrat(-/-) cones before the onset of massive ventral/central cone degeneration.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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