Full Product Name
RDH8 siRNA (Human)
Product Synonym Names
PRRDH; Retinol dehydrogenase 8; Photoreceptor outer segment all-trans retinol dehydrogenase
Product Gene Name
RDH8 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9NYR8
Specificity
RDH8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RDH8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of RDH8 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
RDH8 sirna
siRNA to inhibit RDH8 expression using RNA interference
Applications Tested/Suitable for RDH8 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for RDH8. It may not necessarily be applicable to this product.
NCBI Accession #
NP_056540.2
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NCBI GenBank Nucleotide #
NM_015725.2
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UniProt Primary Accession #
Q9NYR8
[Other Products]
UniProt Secondary Accession #
Q9H838[Other Products]
UniProt Related Accession #
Q9NYR8[Other Products]
Molecular Weight
33,755 Da
NCBI Official Full Name
retinol dehydrogenase 8
NCBI Official Synonym Full Names
retinol dehydrogenase 8 (all-trans)
NCBI Official Symbol
RDH8??[Similar Products]
NCBI Official Synonym Symbols
PRRDH; SDR28C2
??[Similar Products]
NCBI Protein Information
retinol dehydrogenase 8
UniProt Protein Name
Retinol dehydrogenase 8
UniProt Synonym Protein Names
Photoreceptor outer segment all-trans retinol dehydrogenase; Short chain dehydrogenase/reductase family 28C member 2
Protein Family
Retinol dehydrogenase
UniProt Gene Name
RDH8??[Similar Products]
UniProt Synonym Gene Names
PRRDH; SDR28C2??[Similar Products]
UniProt Entry Name
RDH8_HUMAN
NCBI Summary for RDH8
This gene encodes a member of the short-chain dehydrogenase/reductase family. The encoded protein catalyzes the reduction of all-trans-retinal to all-trans-retinol, the first reaction step of the rhodopsin regeneration pathway. This enzymatic reaction is the rate-limiting step in the visual cycle. [provided by RefSeq, Feb 2014]
UniProt Comments for RDH8
RDH8: Retinol dehydrogenase with a clear preference for NADP. Converts all-trans-retinal to all-trans-retinol. May play a role in the regeneration of visual pigment at high light intensity. Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Protein type: Cofactor and Vitamin Metabolism - retinol; EC 1.1.1.300; Membrane protein, integral; Oxidoreductase; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: integral to plasma membrane; cytoplasm
Molecular Function: estradiol 17-beta-dehydrogenase activity; retinol dehydrogenase activity
Biological Process: phototransduction, visible light; visual perception; retinol metabolic process; retinoid metabolic process; steroid biosynthetic process; estrogen biosynthetic process
Research Articles on RDH8
1. Progression of retinal degeneration in Rdh8-deficient Abca4-deficient mice is affected by differential vulnerability of rods and cones to light.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
