Full Product Name
GJB6 siRNA (Human)
Product Synonym Names
Gap junction beta-6 protein; Connexin-30; Cx30
Product Gene Name
GJB6 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O95452
Specificity
GJB6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GJB6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GJB6 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GJB6 sirna
siRNA to inhibit GJB6 expression using RNA interference
Applications Tested/Suitable for GJB6 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GJB6. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001103689.1
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NCBI GenBank Nucleotide #
NM_001110219.2
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UniProt Primary Accession #
O95452
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UniProt Secondary Accession #
Q5Q1H9; Q5Q1I0; Q5Q1I1; Q5T5U0; Q8IUP0; B3KQN2[Other Products]
UniProt Related Accession #
O95452[Other Products]
Molecular Weight
30,387 Da
NCBI Official Full Name
gap junction beta-6 protein
NCBI Official Synonym Full Names
gap junction protein, beta 6, 30kDa
NCBI Official Symbol
GJB6??[Similar Products]
NCBI Official Synonym Symbols
ED2; EDH; HED; CX30; HED2; DFNA3; ECTD2; DFNA3B; DFNB1B
??[Similar Products]
NCBI Protein Information
gap junction beta-6 protein
UniProt Protein Name
Gap junction beta-6 protein
UniProt Synonym Protein Names
Connexin-30; Cx30
Protein Family
Gap junction beta-6 protein
UniProt Gene Name
GJB6??[Similar Products]
UniProt Synonym Gene Names
Cx30??[Similar Products]
UniProt Entry Name
CXB6_HUMAN
NCBI Summary for GJB6
Gap junctions allow the transport of ions and metabolites between the cytoplasm of adjacent cells. They are formed by two hemichannels, made up of six connexin proteins assembled in groups. Each connexin protein has four transmembrane segments, two extracellular loops, a cytoplasmic loop formed between the two inner transmembrane segments, and the N- and C-terminus both being in the cytoplasm. The specificity of the gap junction is determined by which connexin proteins comprise the hemichannel. In the past, connexin protein names were based on their molecular weight, however the new nomenclature uses sequential numbers based on which form (alpha or beta) of the gap junction is present. This gene encodes one of the connexin proteins. Mutations in this gene have been found in some forms of deafness and in some families with hidrotic ectodermal dysplasia. [provided by RefSeq, Jul 2008]
UniProt Comments for GJB6
GJB6: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Belongs to the connexin family. Beta-type (group I) subfamily. A connexon is composed of a hexamer of connexins. Interacts with CNST
Protein type: Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 13q12
Cellular Component: connexon complex; apical plasma membrane; integral to membrane; cytosol
Biological Process: negative regulation of cell proliferation; sensory perception of sound; apoptosis; cell communication; response to lipopolysaccharide; ear morphogenesis; response to electrical stimulus; inner ear development; aging
Disease: Deafness, Autosomal Recessive 1a; Clouston Syndrome; Deafness, Autosomal Recessive 1b; Deafness, X-linked 2; Deafness, Autosomal Dominant 3b
Research Articles on GJB6
1. Mutations in GJB2, GJB6 and GJA1 are not a major cause of non-syndromic deafness in black Africans
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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