Product Name
CLCN5, siRNA
Full Product Name
CLCN5 siRNA (Mouse)
Product Synonym Names
CLC5; H(+)/Cl(-) exchange transporter 5; Chloride channel protein 5; ClC-5; Chloride transporter ClC-5
Product Gene Name
CLCN5 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9WVD4
Specificity
CLCN5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CLCN5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CLCN5 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CLCN5 sirna
siRNA to inhibit CLCN5 expression using RNA interference
Applications Tested/Suitable for CLCN5 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CLCN5. It may not necessarily be applicable to this product.
NCBI Accession #
NP_057900.3
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NCBI GenBank Nucleotide #
NM_016691.4
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UniProt Primary Accession #
Q9WVD4
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UniProt Secondary Accession #
B1AXN0[Other Products]
UniProt Related Accession #
Q9WVD4[Other Products]
Molecular Weight
83,101 Da
NCBI Official Full Name
H(+)/Cl(-) exchange transporter 5 isoform 1
NCBI Official Synonym Full Names
chloride channel 5
NCBI Official Symbol
Clcn5??[Similar Products]
NCBI Official Synonym Symbols
Clc5; ClC-5; Sfc13; T25545; DXImx42e; 5430408K11Rik; D930009B12Rik
??[Similar Products]
NCBI Protein Information
H(+)/Cl(-) exchange transporter 5
UniProt Protein Name
H(+)/Cl(-) exchange transporter 5
UniProt Synonym Protein Names
Chloride channel protein 5; ClC-5; Chloride transporter ClC-5
Protein Family
H(+)/Cl(-) exchange transporter
UniProt Gene Name
Clcn5??[Similar Products]
UniProt Synonym Gene Names
Clc5; ClC-5??[Similar Products]
UniProt Entry Name
CLCN5_MOUSE
UniProt Comments for CLCN5
CLCN5: Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function. Defects in CLCN5 are a cause of hypophosphatemic rickets, X-linked recessive (XLRHR). XLRHR is a renal disease belonging to the 'Dent disease complex', a group of disorders characterized by proximal renal tubular defect, hypercalciuria, nephrocalcinosis, and renal insufficiency. The spectrum of phenotypic features is remarkably similar in the various disorders, except for differences in the severity of bone deformities and renal impairment. XLRH patients present with rickets or osteomalacia, hypophosphatemia due to decreased renal tubular phosphate reabsorption, hypercalciuria, and low molecular weight proteinuria. Patients develop nephrocalcinosis with progressive renal failure in *****hood. Female carriers may have asymptomatic hypercalciuria or hypophosphatemia only. Defects in CLCN5 are the cause of nephrolithiasis type 2 (NPHL2); also known as Dent disease 1. NPHL2 is an X- linked recessive renal disease belonging to the 'Dent disease complex'. NPHL2 patients manifest hypercalciuria, hypophosphatemia, aminoaciduria, nephrocalcinosis and nephrolithiasis, renal insufficiency leading to renal failure in *****hood, rickets (33% of patients) and osteomalacia. Defects in CLCN5 are the cause of nephrolithiasis type 1 (NPHL1); also designated XRN. NPHL1 is an X-linked recessive renal disease belonging to the 'Dent disease complex'. NPHL1 presents with hypercalciuria, nephrocalcinosis, renal stones and renal insufficiency. Patients lack urinary acidification defects, rickets, and osteomalacia. Defects in CLCN5 are the cause of low molecular weight proteinuria with hypercalciuria and nephrocalcinosis (LMWPHN). LMWPHN is an X-linked renal disease belonging to the 'Dent disease complex'. Patients tend to have hypercalciuric nephrocalcinosis without rickets or renal failure. Belongs to the chloride channel (TC 2.A.49) family. ClC-5/CLCN5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter; Membrane protein, integral; Membrane protein, multi-pass; Transporter, ion channel
Cellular Component: Golgi apparatus; membrane; lysosomal membrane; apical part of cell; integral to membrane; plasma membrane; endosome
Molecular Function: nucleotide binding; ion channel activity; voltage-gated chloride channel activity; ATP binding; antiporter activity
Biological Process: transport; endocytosis; chloride transport; ion transport; transmembrane transport
Research Articles on CLCN5
1. This study demonistrated that Clcn5 gene expression in mouse dorsal raphe nucleus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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