Gap junction beta-6 protein; Connexin-30; Cx30; GJB6

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This GJB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-117 amino acids from the N-terminal region of human GJB6.

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Vial Concentration: 1.1 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-GJB6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) play crucial functional roles associated with these channels. I Mutations in GJB2 are associated with genetically derived hearing impairments, including autosomal dominant, bilateral, middle to high frequency hearing loss.

Neuroscience; Signal Transduction

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

WB~~1:1000

Western blot analysis of GJB6 (arrow) using rabbit polyclonal GJB6 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GJB6gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with GJB6 Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

30387

gap junction protein, beta 6, 30kDa

gap junction beta-6 protein

Gap junction beta-6 protein

Cx30??[Similar Products]

CXB6_HUMAN

Gap junctions allow the transport of ions and metabolites between the cytoplasm of adjacent cells. They are formed by two hemichannels, made up of six connexin proteins assembled in groups. Each connexin protein has four transmembrane segments, two extracellular loops, a cytoplasmic loop formed between the two inner transmembrane segments, and the N- and C-terminus both being in the cytoplasm. The specificity of the gap junction is determined by which connexin proteins comprise the hemichannel. In the past, connexin protein names were based on their molecular weight, however the new nomenclature uses sequential numbers based on which form (alpha or beta) of the gap junction is present. This gene encodes one of the connexin proteins. Mutations in this gene have been found in some forms of deafness and in some families with hidrotic ectodermal dysplasia. [provided by RefSeq, Jul 2008]

GJB6: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Belongs to the connexin family. Beta-type (group I) subfamily. A connexon is composed of a hexamer of connexins. Interacts with CNST

Protein type: Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: connexon complex; apical plasma membrane; integral to membrane; cytosol

Biological Process: negative regulation of cell proliferation; sensory perception of sound; apoptosis; response to lipopolysaccharide; cell communication; ear morphogenesis; response to electrical stimulus; inner ear development; aging

Disease: Deafness, Autosomal Recessive 1a; Clouston Syndrome; Deafness, Autosomal Recessive 1b; Deafness, X-linked 2; Deafness, Autosomal Dominant 3b

Beltramello, M., et al., Biochem. Biophys. Res. Commun. 305(4):1024-1033 (2003).
Common, J.E., et al., Biochem. Biophys. Res. Commun. 298(5):651-656 (2002).
Smith, F.J., et al., J. Invest. Dermatol. 118(3):530-532 (2002).
del Castillo, I., et al., N. Engl. J. Med. 346(4):243-249 (2002).
Pallares-Ruiz, N., et al., Eur. J. Hum. Genet. 10(1):72-76 (2002).

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