Product Name
MLKL, Polyclonal Antibody
Popular Item
Full Product Name
MLKL Polyclonal Antibody
Product Synonym Names
9130019I15Rik
Product Gene Name
anti-MLKL antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9D2Y4
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human MLKL
Calculated Molecular Weight
54kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-MLKL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-MLKL antibody
Polyclonal
Applications Tested/Suitable for anti-MLKL antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-MLKL antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Western Blot (WB) of anti-MLKL antibody
Western blot analysis of extracts of various cell lines, using MLKL antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
NCBI/Uniprot data below describe general gene information for MLKL. It may not necessarily be applicable to this product.
NCBI Accession #
Q9D2Y4.1
[Other Products]
UniProt Primary Accession #
Q9D2Y4
[Other Products]
UniProt Secondary Accession #
Q8CIJ5[Other Products]
UniProt Related Accession #
Q9D2Y4[Other Products]
NCBI Official Full Name
Mixed lineage kinase domain-like protein
NCBI Official Synonym Full Names
mixed lineage kinase domain-like
NCBI Official Symbol
Mlkl??[Similar Products]
NCBI Official Synonym Symbols
9130019I15Rik
??[Similar Products]
NCBI Protein Information
mixed lineage kinase domain-like protein; mlkl
UniProt Protein Name
Mixed lineage kinase domain-like protein
Protein Family
Mixed lineage kinase domain-like protein
UniProt Gene Name
Mlkl??[Similar Products]
UniProt Entry Name
MLKL_MOUSE
NCBI Summary for MLKL
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]
UniProt Comments for MLKL
MLKL: Belongs to the protein kinase superfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; TKL-Unique family
Cellular Component: membrane; cytoplasm; plasma membrane
Molecular Function: transferase activity, transferring phosphorus-containing groups; protein complex binding; nucleotide binding; protein kinase binding; ATP binding; protein kinase activity
Biological Process: programmed cell death; protein amino acid phosphorylation
Research Articles on MLKL
1. These results indicate that necroptosis caused by RIPK3 requires MLKL but not Drp1.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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