Full Product Name
Mouse Monoclonal [clone 3B2] (IgG2a,k) to Human MLKL
Product Synonym Names
Anti-MLKL Antibody (clone 3B2) IHC-plus; MLKL; Human MLKL
Product Gene Name
anti-MLKL antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8NB16
Purity/Purification
Protein A Purified
Concentration
1 mg/ml (lot specific)
Immunogen Description
MLKL (NP_689862, 371 a.a. ~ 472 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
MLKL antibody was raised against mLKL (NP_689862, 371 a.a. ~ 472 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Other Notes
Small volumes of anti-MLKL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-MLKL antibody
Subfamily:-Provisional-protein-kinase">Family: Protein Kinase
Subfamily: Provisional protein kinase
Applications Tested/Suitable for anti-MLKL antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-MLKL antibody
ELISA, IHC-P (10 ug/ml), WB
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.
Immunohistochemistry (IHC) of anti-MLKL antibody
Anti-MLKL antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
ELISA (EIA) of anti-MLKL antibody
Detection limit for recombinant GST tagged MLKL is 0.3 ng/ml as a capture antibody.
NCBI/Uniprot data below describe general gene information for MLKL. It may not necessarily be applicable to this product.
NCBI Accession #
NP_689862.1
[Other Products]
NCBI GenBank Nucleotide #
NM_152649.2
[Other Products]
UniProt Primary Accession #
Q8NB16
[Other Products]
UniProt Secondary Accession #
Q8N6V0; A6NCE4[Other Products]
UniProt Related Accession #
Q8NB16[Other Products]
Molecular Weight
30,279 Da
NCBI Official Full Name
mixed lineage kinase domain-like protein isoform 1
NCBI Official Synonym Full Names
mixed lineage kinase domain-like
NCBI Official Symbol
MLKL??[Similar Products]
NCBI Official Synonym Symbols
hMLKL
??[Similar Products]
NCBI Protein Information
mixed lineage kinase domain-like protein; MLKL
UniProt Protein Name
Mixed lineage kinase domain-like protein
Protein Family
Mixed lineage kinase domain-like protein
UniProt Gene Name
MLKL??[Similar Products]
UniProt Synonym Gene Names
hMLKL??[Similar Products]
UniProt Entry Name
MLKL_HUMAN
NCBI Summary for MLKL
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]
UniProt Comments for MLKL
MLKL: Belongs to the protein kinase superfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; TKL-Unique family
Chromosomal Location of Human Ortholog: 16q23.1
Cellular Component: cytoplasm; plasma membrane
Molecular Function: protein binding; protein complex binding; protein kinase binding; ATP binding; protein kinase activity
Biological Process: protein amino acid phosphorylation
Research Articles on MLKL
1. MLKL binding to phosphatidylinositol phosphates is required for plasma membrane rupture
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
